Modified interferon beta polypeptides and their uses

• US 8,114,630 B2
• Filed: 04/30/2008
• Issued: 02/14/2012
• Est. Priority Date: 05/02/2007
• Status: Expired due to Fees

• Current Assignee: Ambrx, Inc. (Johnson & Johnson)
• Original Assignee: Ambrx, Inc. (Johnson & Johnson)
• Inventors: Kraynov, Vadim, Knudsen, Nick, Hays Putnam, Anna-Maria A., Krawitz, Denise, Pinkstaff, Jason, Myler, Heather
• Primary Examiner(s): Saoud, Christine J
• Assistant Examiner(s): Seharaseyon, Jegatheesan
• Application Number:
  US12/112,890
• Publication Number:
  US 20090208454A1
• Time in Patent Office: 1,385 Days
• Field of Search: None
• US Class Current: 435/69.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 13/10 of the bladder
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 15/00 Drugs for genital or sexual disorders for disorders of sex hormones A61P5/24; Contraceptives
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: C07K 14/565 IFN-beta

Abstract

Modified interferon beta polypeptides and uses thereof are provided.

Claims

1. An isolated nucleic acid comprising a polynucleotide that encodes an interferon beta polypeptide comprising an amino acid sequence SEQ ID NO:

1, SEQ ID NO;

3 or SEQ ID NO;

4, wherein the polynucleotide comprises an amber selector codon and wherein the amber selector codon selectively incorporates a non-naturally encoded amino acid at amino acid position 36 of the polypeptide sequence of SEQ ID NO;

1 or SEQ ID NO;

3, or the corresponding amino acid position in SEQ ID NO;

4, and wherein the non-naturally encoded amino acid has a ketone moiety.

2. A cell comprising the nucleic acid of claim 1.

3. The cell of claim 2, wherein the cell comprises an orthogonal tRNA synthetase or an orthogonal tRNA.

4. A method of making a IFN beta polypeptide comprising a non-naturally encoded amino acid, the method comprising, culturing cells comprising a polynucleotide or polynucleotides encoding a IFN beta polypeptide comprising an amber selector codon wherein the amber codon is selective for a non-naturally encoded amino acid with a ketone functional group, an orthogonal RNA synthetase and an orthogonal tRNA under conditions to permit expression of the IFN beta polypeptide comprising a non-naturally encoded amino acid at amino acid position 36 of polypeptide sequence SEQ ID NO:

1 or the corresponding amino acids in SEQ ID NOs;

3 or 4; and

purifying the IFN beta polypeptide.