Modified interferon beta polypeptides and their uses
First Claim
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1. An isolated nucleic acid comprising a polynucleotide that encodes an interferon beta polypeptide comprising an amino acid sequence SEQ ID NO:
- 1, SEQ ID NO;
3 or SEQ ID NO;
4, wherein the polynucleotide comprises an amber selector codon and wherein the amber selector codon selectively incorporates a non-naturally encoded amino acid at amino acid position 36 of the polypeptide sequence of SEQ ID NO;
1 or SEQ ID NO;
3, or the corresponding amino acid position in SEQ ID NO;
4, and wherein the non-naturally encoded amino acid has a ketone moiety.
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Abstract
Modified interferon beta polypeptides and uses thereof are provided.
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Citations
4 Claims
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1. An isolated nucleic acid comprising a polynucleotide that encodes an interferon beta polypeptide comprising an amino acid sequence SEQ ID NO:
- 1, SEQ ID NO;
3 or SEQ ID NO;
4, wherein the polynucleotide comprises an amber selector codon and wherein the amber selector codon selectively incorporates a non-naturally encoded amino acid at amino acid position 36 of the polypeptide sequence of SEQ ID NO;
1 or SEQ ID NO;
3, or the corresponding amino acid position in SEQ ID NO;
4, and wherein the non-naturally encoded amino acid has a ketone moiety. - View Dependent Claims (2, 3)
- 1, SEQ ID NO;
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4. A method of making a IFN beta polypeptide comprising a non-naturally encoded amino acid, the method comprising, culturing cells comprising a polynucleotide or polynucleotides encoding a IFN beta polypeptide comprising an amber selector codon wherein the amber codon is selective for a non-naturally encoded amino acid with a ketone functional group, an orthogonal RNA synthetase and an orthogonal tRNA under conditions to permit expression of the IFN beta polypeptide comprising a non-naturally encoded amino acid at amino acid position 36 of polypeptide sequence SEQ ID NO:
- 1 or the corresponding amino acids in SEQ ID NOs;
3 or 4; and
purifying the IFN beta polypeptide.
- 1 or the corresponding amino acids in SEQ ID NOs;
Specification