Electronic methods for the detection of analytes

• US 8,114,661 B2
• Filed: 05/04/2010
• Issued: 02/14/2012
• Est. Priority Date: 06/12/1997
• Status: Expired due to Fees

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: O'Connor, Stephen D., Kayyem, Jon Faiz, Meade, Thomas J.
• Primary Examiner(s): Cook, Lisa
• Application Number:
  US12/773,382
• Publication Number:
  US 20110059535A1
• Time in Patent Office: 651 Days
• Field of Search: None
• US Class Current: 435/287.9
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2458/30 Electrochemically active labels
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 2800/04 Endocrine or metabolic disorders
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The present invention is directed to the detection of target analytes using electronic techniques, particularly AC techniques.

Claims

1. A method of detecting a protein target analyte in a sample comprising:

a) applying a first input signal to an assay complex comprising;

i) a redox active complex comprising;

1) a redox active molecule;

2) a binding ligand that will bind said protein target analyte; and

ii) said protein target analyte,

 

wherein said binding ligand is covalently attached to a first measuring electrode via a spacer, wherein said spacer is attached to said electrode with more than one sulfur moiety, and wherein said first measuring electrode further comprises a passivation agent monolayer comprising at least a covalently attached first passivation species; and

b) detecting a change in the faradaic impedance of the system as a result of the association of the redox active molecule with said protein target analyte, if present.

2. A method according to claim 1 wherein said protein target analyte is an enzyme.

3. A method according to claim 1 wherein said binding ligand is a peptide.

4. A method according to claim 1 wherein said redox active molecule is a transition metal complex.

5. A method according to claim 4 wherein said transition metal complex comprises a transition metal selected from the group consisting of iron, ruthenium and osmium.

6. A method according to claim 4 wherein said transitional metal complex is ferrocene.

7. A method according to claim 1 wherein said spacer is a conductive oligomer.

8. A method according to claim 1 wherein said passivation species is an insulator.