Method of detection of nucleic acids with a specific sequence composition
First Claim
1. An isolated target binding assembly (TBA) comprising a first and a second nucleic acid recognition unit, wherein:
- said first nucleic acid recognition unit is capable of binding with specificity to a first target nucleotide sequence contained within a target polynucleotide;
said second nucleic acid recognition unit is capable of binding with specificity to a second target nucleotide sequence contained within a target polynucleotide;
said nucleic acid recognition units are polypeptides and are the same or different; and
wherein said TBA comprises a sequence selected from the group consisting of;
A. I+II+III;
B. IV+V+III; and
C. IV+III;
whereinI indicates any of SEQ ID NOS;
85-92;
II indicates Met Ser, linked to any of SEQ ID NOS;
104-106, each of which is linked to SEQ ID NO;
99;
III indicates SEQ ID NO;
100 linked to any of SEQ ID NOS;
75-84 or 94-98;
SEQ ID NO;
101 linked to either SEQ ID NO;
74 or SEQ ID NO;
93;
or SEQ ID NO;
102 linked to SEQ ID NO;
74 or SEQ ID NO;
93;
or any of SEQ ID NOS;
72, 103, 73, or 63-71;
IV indicates any of SEQ ID NOS. 104-106; and
V indicates SEQ ID NO;
99.
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Accused Products
Abstract
This invention is a novel method for detecting and localizing specific nucleic acid sequences in a sample with a high degree of sensitivity and specificity. The method and novel compositions used in the method involve the use of Probe Nucleic Acids, the production of nucleic acid binding regions and the use of nucleic acid Target Binding Assemblies to detect and localize specific Target Nucleic Acids. The detection and localization of the Target Nucleic Acid is accomplished even in the presence of nucleic acids which have similar sequences. The method provides for a high degree of amplification of the signal produced by each specific binding event. In particular, methods and compositions are presented for the detection of HIV and HPV nucleic acid in samples. These methods and compositions find use in diagnosis of disease, genetic monitoring, forensics, and analysis of nucleic acid mixtures. Some of the novel compositions used in the detection method are useful in preventing or treating pathogenic conditions.
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Citations
9 Claims
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1. An isolated target binding assembly (TBA) comprising a first and a second nucleic acid recognition unit, wherein:
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said first nucleic acid recognition unit is capable of binding with specificity to a first target nucleotide sequence contained within a target polynucleotide; said second nucleic acid recognition unit is capable of binding with specificity to a second target nucleotide sequence contained within a target polynucleotide; said nucleic acid recognition units are polypeptides and are the same or different; and wherein said TBA comprises a sequence selected from the group consisting of; A. I+II+III; B. IV+V+III; and C. IV+III; wherein I indicates any of SEQ ID NOS;
85-92;II indicates Met Ser, linked to any of SEQ ID NOS;
104-106, each of which is linked to SEQ ID NO;
99;III indicates SEQ ID NO;
100 linked to any of SEQ ID NOS;
75-84 or 94-98;
SEQ ID NO;
101 linked to either SEQ ID NO;
74 or SEQ ID NO;
93;
or SEQ ID NO;
102 linked to SEQ ID NO;
74 or SEQ ID NO;
93;
or any of SEQ ID NOS;
72, 103, 73, or 63-71;IV indicates any of SEQ ID NOS. 104-106; and V indicates SEQ ID NO;
99.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification