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Method of detection of nucleic acids with a specific sequence composition

  • US 8,114,962 B2
  • Filed: 06/29/2009
  • Issued: 02/14/2012
  • Est. Priority Date: 12/09/1994
  • Status: Expired due to Fees
First Claim
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1. An isolated target binding assembly (TBA) comprising a first and a second nucleic acid recognition unit, wherein:

  • said first nucleic acid recognition unit is capable of binding with specificity to a first target nucleotide sequence contained within a target polynucleotide;

    said second nucleic acid recognition unit is capable of binding with specificity to a second target nucleotide sequence contained within a target polynucleotide;

    said nucleic acid recognition units are polypeptides and are the same or different; and

    wherein said TBA comprises a sequence selected from the group consisting of;

    A. I+II+III;

    B. IV+V+III; and

    C. IV+III;

    whereinI indicates any of SEQ ID NOS;

    85-92;

    II indicates Met Ser, linked to any of SEQ ID NOS;

    104-106, each of which is linked to SEQ ID NO;

    99;

    III indicates SEQ ID NO;

    100 linked to any of SEQ ID NOS;

    75-84 or 94-98;

    SEQ ID NO;

    101 linked to either SEQ ID NO;

    74 or SEQ ID NO;

    93;

    or SEQ ID NO;

    102 linked to SEQ ID NO;

    74 or SEQ ID NO;

    93;

    or any of SEQ ID NOS;

    72, 103, 73, or 63-71;

    IV indicates any of SEQ ID NOS. 104-106; and

    V indicates SEQ ID NO;

    99.

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