Di-alpha amino anthraquinone compositions
First Claim
1. A compound comprising an oligonucleotide labeled with a fluorophore and quencher, wherein the oligonucleotide is adapted to hybridize to a selected nucleic acid sequence, and wherein the quencher is a di-alpha amino anthraquinone comprising a first amino group and a second amino group attached at alpha-positions of the anthraquinone, wherein fluorescence of the fluorophore is reduced by fluorescence resonance energy transfer to the quencher or by ground state quenching by the quencher when the oligonucleotide is not hybridized to the selected nucleic acid sequence.
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Abstract
The invention provides novel anthraquinone compositions that are useful as broad-spectrum quenchers of fluorescence and provides methods for making and using them. The anthraquinone quenchers can be conjugated to a variety of biologically relevant compounds, including lipids, nucleic acids, polypeptides, and more specifically antigens, steroids, vitamins, drugs, haptens, metabolites, toxins, environmental pollutants, amino acids, peptides, proteins, nucleotides, oligonucleotides, polynucleotides, carbohydrates, and their analogs. The invention also provides kits comprising, in one or more containers, at least one anthraquinone quencher dye composition of the present invention, and instructions for using that composition.
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6 Claims
- 1. A compound comprising an oligonucleotide labeled with a fluorophore and quencher, wherein the oligonucleotide is adapted to hybridize to a selected nucleic acid sequence, and wherein the quencher is a di-alpha amino anthraquinone comprising a first amino group and a second amino group attached at alpha-positions of the anthraquinone, wherein fluorescence of the fluorophore is reduced by fluorescence resonance energy transfer to the quencher or by ground state quenching by the quencher when the oligonucleotide is not hybridized to the selected nucleic acid sequence.
Specification