Compositions and use of EPAS1 inhibitors
First Claim
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1. A nucleic acid comprising:
- (i) a first strand of about 15 to about 30 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39 and 41, and(ii) a second strand of about 15 to about 30 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32,34, 36, 38, 40, and 42,wherein at least 12 nucleotides of the first and second strands are complementary to each other and form a double-stranded nucleic acid under physiological conditions, and wherein the double-stranded nucleic acid can reduce the expression of endothelial PAS domain protein 1 (EPAS1) in a cell by an RNA interference mechanism.
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Abstract
The present invention relates to inhibitors of endothelial PAS domain protein 1 (EPAS1), and methods and compositions related to the EPAS1 inhibitors. In certain embodiments, the EPAS1 inhibitors include nucleic acids, such as for example siRNAs.
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Citations
39 Claims
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1. A nucleic acid comprising:
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(i) a first strand of about 15 to about 30 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39 and 41, and(ii) a second strand of about 15 to about 30 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32,34, 36, 38, 40, and 42,wherein at least 12 nucleotides of the first and second strands are complementary to each other and form a double-stranded nucleic acid under physiological conditions, and wherein the double-stranded nucleic acid can reduce the expression of endothelial PAS domain protein 1 (EPAS1) in a cell by an RNA interference mechanism. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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12. An isolated nucleic acid comprising a sequence that hybridizes to a region of an EPAS1 transcript corresponding to nucleotides 655-718 of SEQ ID NO:
- 1 under physiological conditions and decreases the expression of EPAS 1 in a cell, comprising a first strand of about 15 to about 50 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39 and 41, and a second strand of about 15 to about 30 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, and 42. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27)
- 1 under physiological conditions and decreases the expression of EPAS 1 in a cell, comprising a first strand of about 15 to about 50 nucleotides in length that comprises a sequence selected from SEQ ID NOs;
Specification