Methods, arrangements and systems for obtaining information associated with a sample using optical microscopy

• US 8,115,919 B2
• Filed: 05/02/2008
• Issued: 02/14/2012
• Est. Priority Date: 05/04/2007
• Status: Active Grant

• Current Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Original Assignee: The General Hospital Corporation (Mass General Brigham, Inc.)
• Inventors: Yun, Seok-Hyun, Scarcelli, Giuliano
• Primary Examiner(s): Chowdhury, Tarifur
• Assistant Examiner(s): Nur, Abdullahi
• Application Number:
  US12/114,081
• Publication Number:
  US 20090323056A1
• Time in Patent Office: 1,383 Days
• Field of Search: 356/301, 356 72- 73, 356/326
• US Class Current: 356/301
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/4412 Scattering spectrometry particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26

Abstract

Exemplary embodiments of methods, arrangements and systems for obtaining information about a sample can be provided. For example, in one exemplary embodiment, it is possible to receive a first electro-magnetic radiation from a sample which is based on a second electro-magnetic radiation forwarded to the sample. The first electro-magnetic radiation may have a first frequency and the second electro-magnetic radiation may have a second frequency which is different from the first frequency. The difference between the first and second frequencies can be based on an acoustic wave inside the sample related to at least one characteristic of the sample. Further, it is possible to receive at least a portion of the first electromagnetic radiation and separate it into a particular finite number (N) of frequency component radiations. In addition, it is possible to receive a particular energy of more than 1/N of energy of the third electro-magnetic radiation, and generate information associated with the sample. Certain exemplary embodiments of the present invention are capable of obtaining information associated with a sample, particularly its mechanical properties, non-contact using electromagnetic radiation.

Claims

1. An apparatus comprising:

at least one first arrangement configured to receive at least one first electro-magnetic radiation provided from a sample which is based on at least one second electro-magnetic radiation forwarded to the sample, wherein the at least one first electro-magnetic radiation has a first frequency and the at least one second electro-magnetic radiation has a second frequency which is different from the first frequency, and wherein a difference between the first and second frequencies is based on an acoustic wave inside the sample related to at least one characteristic of the sample;

at least one second arrangement configured to receive at least one third electro-magnetic radiation from the at least one first arrangement which is based on the at least one first electro-magnetic radiation and separate the at least one third electro-magnetic radiation into a particular finite number (N) of frequency component radiations; and

at least one third arrangement configured to receive a particular energy of more than 1/N of energy of the at least one third electro-magnetic radiation, and generate information associated with the sample.

2. The apparatus according to claim 1, wherein the at least one third arrangement is configured to receive the particular energy of at least one particular radiation of the frequency component radiations.

3. The apparatus according to claim 1, wherein the at least one third arrangement includes an array of detectors.

4. The apparatus according to claim 1, wherein the at least one second arrangement includes a virtually imaged phased array.

5. The apparatus according to claim 1, wherein the particular number is between about 10 and 100.

6. The apparatus according to claim 1, wherein the at least one third arrangement is configured to simultaneously receive a plurality of the frequency component radiations.

7. The apparatus according to claim 1, wherein the at least one first arrangement includes a narrow-band spectral filter arrangement which receives the at least one first electro-magnetic radiation and attenuates at least one portion thereof which has a frequency which is approximately the same as the second frequency.

8. The apparatus according to claim 1, wherein the at least one first arrangement includes a single-mode optical fiber arrangement.

9. The apparatus according to claim 1, wherein the at least one first arrangement includes a confocal arrangement.

10. The apparatus according to claim 1, wherein a resolvable linewidth of at least one frequency component radiation is less than 2 GHz.

11. The apparatus according to claim 1, further comprising at least one fourth arrangement configured to image the at least one portion of the sample based on the information.

12. The apparatus according to claim 11, wherein the information includes a difference between a frequency of at least one of the frequency component radiations and the second frequency.

13. The apparatus according to claim 12, wherein the difference is between approximately −

100 GHz and +100 GHz.

14. The apparatus according to claim 11, wherein the information includes an energy of each of the frequency component radiations.

15. The apparatus according to claim 11, wherein the information includes a linewidth of a plurality of the frequency component radiations each of which being associated with a particular frequency.

16. The apparatus according to claim 11, wherein the information includes at least one characteristic of the sample.

17. The apparatus according to claim 16, wherein the at least one characteristic includes at least one bio-mechanical property of the sample.

18. The apparatus according to claim 17, wherein the at least one bio-mechanical property includes complex viscoelastic modulus.

19. The apparatus according to claim 16, wherein the at least one characteristic includes a crosslinking of collagen in the sample.

20. The apparatus according to claim 11, wherein the sample includes at least one of an anatomical structure or a biomaterial.

21. The apparatus according to claim 1, further comprising at least one fifth arrangement configured to deliver the at least one second electro-magnetic radiation to the sample.

22. The apparatus according to claim 21, wherein the at least one fifth arrangement scans the sample by moving the at least one second electro-magnetic radiation across the sample.

23. The apparatus according to claim 21, wherein the at least one fifth arrangement includes a fiber optic arrangement.

24. An apparatus comprising:

at least one first arrangement configured to receive at least one first electro-magnetic radiation and at least one second electro-magnetic radiation provided from a sample, wherein the first and second electro-magnetic radiations which are based on at least one third electro-magnetic radiation forwarded to the sample, wherein the at least one first electro-magnetic radiation has a first frequency, the at least one second electro-magnetic radiation has a second frequency and the at least one third electro-magnetic radiation has a third frequency which is different from the first frequency, wherein a difference between the first and third frequencies is based on an acoustic wave inside the sample related to at least one characteristic of the sample, wherein the at least one first arrangement is further configured to separate the first and second electro-magnetic radiations into first and second respective frequency component radiations; and

at least one second arrangement configured to simultaneously detect the first and second frequency component radiations, and generate information associated with the sample based on the first and second frequency component radiations.

25. The apparatus according to claim 24, wherein the at least one second electro-magnetic radiation is based on at least one of an elastic scattering, a vibrational Raman scattering or a fluorescence within the sample.

26. The apparatus according to claim 24, wherein the difference between the at least one first frequency and the at least one third frequency is greater than 10 THz.

27. The apparatus according to claim 24, further comprising at least one third arrangement configured to image the at least one portion of the sample based on the information.

28. The apparatus according to claim 24, wherein the at least one first arrangement includes a narrow band spectral separating arrangement for separating the at least one first electro-magnetic radiation, and a broadband spectral separating arrangement for separating the at least one second electro-magnetic radiation.

29. A method comprising:

receiving at least one first electro-magnetic radiation provided from a sample which is based on at least one second electro-magnetic radiation forwarded to the sample, wherein the at least one first electro-magnetic radiation has a first frequency and the at least one second electro-magnetic radiation has a second frequency which is different from the first frequency, and wherein a difference between the first and second frequencies is based on an acoustic wave inside the sample related to at least one characteristic of the sample;

receiving at least one third electro-magnetic radiation which is based on the at least one first electro-magnetic radiation and separate the at least one third electro-magnetic radiation into a particular finite number (N) of frequency component radiations;

receiving a particular energy of more than 1/N of energy of the at least one third electro-magnetic radiation; and

generating information associated with the sample.

30. A method comprising:

receiving at least one first electro-magnetic radiation and at least one second electro-magnetic radiation provided from a sample, wherein the first and second electro-magnetic radiations which are based on at least one third electro-magnetic radiation forwarded to the sample, wherein the at least one first electro-magnetic radiation has a first frequency, the at least one second electro-magnetic radiation has a second frequency and the at least one third electro-magnetic radiation has a third frequency which is different from the first frequency, and wherein a difference between the first and third frequencies is based on an acoustic wave inside the sample related to at least one characteristic of the sample;

separating the first and second electro-magnetic radiations into first and second respective frequency component radiations; and

simultaneously detecting the first and second frequency component radiations, and generate information associated with the sample based on the first and second frequency component radiations.