Nanopatterned surfaces and related methods for selective adhesion, sensing and separation

• US 8,117,902 B2
• Filed: 06/23/2008
• Issued: 02/21/2012
• Est. Priority Date: 11/03/2005
• Status: Active Grant

• Current Assignee: University Of Massachusetts
• Original Assignee: University Of Massachusetts
• Inventors: Santore, Maria Monica, Kalasin, Surachate
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US12/215,046
• Publication Number:
  US 20090124016A1
• Time in Patent Office: 1,338 Days
• Field of Search: 73 2801- 2802, 73/280.4, 73/616.2, 73 6171- 6172, 731/04, 738/655, 738/665, 324/71.1, 324/71.4, 324452-453, 324/464, 435/4, 435/7.1, 435/7.32, 435/7.8, 436/2, 436/86, 977/953, 977956-957
• US Class Current: 73/61.71
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/54306 Solid-phase reaction mechanisms
• CPC Class Codes: G01N 33/54346 Nanoparticles
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/552 Glass or silica
• CPC Class Codes: Y10S 977/953 Detector using nanostructure
• CPC Class Codes: Y10S 977/957 Of chemical property or presence

Abstract

The invention is comprised, in part, of a surface that contains more than one component or construct. Such heterogenous surface compositions and configurations, related systems and methods for sensing particle or analyte interaction therewith can selectively and/or differentially interact with a range of particles/analytes, in lieu of specific molecular sensor-analyte interactions for each particle. These interactions of various analytes or particles can differ sufficiently in strength and range between multiple analyte types or particles to effect a separation of analytes or particles mixtures, in a way that requires no sensing or detection. With incorporation of a sensing mechanism, discrimination/detection of different compounds within an analyte mixture can be accomplished.

Claims

1. A method of using a spatial surface configuration for selective particle interaction, said method comprising:

providing a heterogeneous surface comprising a surface member and a plurality of components thereon, said components spaced about said surface member and having a density thereon, said surface heterogeneity providing different interactions of said surface member and said spaced components with a particle exposed thereto;

exposing a particle to said heterogeneous surface; and

sensing at least one of physical and chemical interaction of said particle with said heterogeneous surface, said interaction selective for said particle.

2. The method of claim 1 wherein said heterogeneity comprises different electrostatic interactions with said particle.

3. The method of claim 2 wherein each of said surface member and said components have a net charge at least partially sufficient for selective particle interaction.

4. The method of claim 3 wherein said components comprise a protein.

5. The method of claim 4 wherein said components comprise an average spatial density, said spatial density at least partially sufficient for selective particle interaction.

6. The method of claim 5 wherein said components comprise a fibrinogen and are spaced an average distance, said distance ranging from about 15 nanometers to about 60 nanometers, from about component center to about component center.

7. The method of claim 5 wherein said protein components are nanodimensioned and spatial density is varied for selective particle interaction.

8. The method of claim 5 wherein said particle has a dimension selected from a spherical radius and a localized surface radius of curvature.

9. The method of claim 8 wherein said spatial density is varied for selective particle interaction.

10. The method of claim 5 wherein said interaction comprises at least one of particle adhesion and particle separation with said surface.

11. The method of claim 10 wherein said exposure comprises a particle flow across said surface and flow rate is varied to affect said particle interaction.

12. The method of claim 11 wherein rate of said particle interaction varies with spatial density.

13. The method of claim 10 comprising sensing a signature interaction of said particle.

14. The method of claim 13 wherein said exposure comprises a mixture of particles, interaction of at least one of said particles selectively sensed with said surface.

15. The method of claim 14 wherein said components comprise an average spatial density, said spatial density at least partially sufficient for selective separation of one said particle.

16. The method of claim 1 wherein said particle is displaced, for another particle exposure to said heterogeneous surface.

17. The method of claim 1 wherein said interaction is selective for separation of said particle.

18. A method for particle sensing, said method comprising:

providing a heterogeneous surface comprising a charged surface member and a plurality of nanodimensioned protein components thereon, said protein components spaced about said surface and having a density thereon, each said protein component comprising a net charge;

exposing a particle to said heterogeneous surface, said particle comprising a net charge; and

sensing interaction of said particle with said heterogeneous surface.

19. The method of claim 18 wherein said protein components comprise an average spatial density, said spatial density at least partially sufficient for selective particle interaction.

20. The method of claim 19 wherein said spatial density is varied for selective particle interaction.

21. The method of claim 19 wherein said protein components are spaced an average distance, said distance ranging from about 15 nanometers to about 60 nanometers, from about component center to about component center.

22. The method of claim 21 wherein said protein components comprise a fibrinogen.

23. The method of claim 18 wherein said exposure comprises a mixture of particles.

24. The method of claim 23 wherein one said particle comprises a first radial dimension and a second said particle comprises a second radial dimension greater than said first radial dimension, each said dimension selected from a spherical radius and a localized surface radius of curvature, said first particle selectively attracted to said heterogeneous surface, said protein components comprising a spatial density at least partially sufficient for selective separation of said first particle from said second particle.

25. The method of claim 23 wherein the ionic strength is varied for selective attraction.

26. The method of claim 18 wherein said particle is displaced, for another particle exposure to said heterogeneous surface.

27. The method of claim 18 wherein said particle comprises a bacterium.

28. The method of claim 18 wherein said interaction is selective for separation of said particle.