Compositions for use in identification of alphaviruses
First Claim
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1. A composition comprising a purified oligonucleotide primer pair configured to generate amplicons from two or more members of the alphavirus genus by hybridizing a forward primer and a reverse primer to conserved regions of a nsP1 encoding gene in two or more members of said alphavirus genus, said primer pair comprising a forward primer with 100% sequence identity with SEQ ID NO:
- 93 and a reverse primer with 100% sequence identity with SEQ ID NO;
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Abstract
The present invention provides oligonucleotide primers and compositions and kits containing the same for rapid identification of alphaviruses by amplification of a segment of viral nucleic acid followed by molecular mass analysis.
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Citations
10 Claims
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1. A composition comprising a purified oligonucleotide primer pair configured to generate amplicons from two or more members of the alphavirus genus by hybridizing a forward primer and a reverse primer to conserved regions of a nsP1 encoding gene in two or more members of said alphavirus genus, said primer pair comprising a forward primer with 100% sequence identity with SEQ ID NO:
- 93 and a reverse primer with 100% sequence identity with SEQ ID NO;
66. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 10)
- 93 and a reverse primer with 100% sequence identity with SEQ ID NO;
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9. A composition comprising a purified oligonucleotide primer pair configured to generate amplicons from two or more members of the alphavirus genus by hybridizing a forward primer and a reverse primer to conserved regions of a nsP1 encoding gene in two or more members of said alphavirus genus, said primer pair comprising a forward primer with 100% sequence identity with SEQ ID NO:
- 21 and a reverse primer with 100% sequence identity with SEQ ID NO;
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- 21 and a reverse primer with 100% sequence identity with SEQ ID NO;
Specification