Biochip analysis system

• US 8,119,391 B2
• Filed: 10/18/2004
• Issued: 02/21/2012
• Est. Priority Date: 05/03/2004
• Status: Expired due to Fees

• Current Assignee: Nanostorage Co., Ltd.
• Original Assignee: Nanostorage Co., Ltd.
• Inventors: Kim, Soo-Kyung, Lee, Seung-Yop, Kim, Kyung-Ho
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US10/595,425
• Publication Number:
  US 20080192588A1
• Time in Patent Office: 2,682 Days
• Field of Search: None
• US Class Current: 435/283.1
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

The present invention relates to a biochip readout device for analyzing and reading out biochips in a rotation manner such that a biochip having been adopted in a non-rotation manner is mounted on an optical disc, and a diagnostic system with a biochip readout device. The biochip readout device comprises biochip cartridge shaped as a rotatable disc, wherein a biochip is installed on or within the disc, a disc rotation drive unit being driven such that the biochip cartridge is rotated, a light reception means for receiving a beam reflected from the disc, the light reception means having a light source scanning the disc with the beam, a focusing/tracking controlling unit for controlling a focusing and tracking operation using the beam received by the light reception means, an optical pick-up unit having an objective lens drive unit for tracking a focus and track of the light source, an optical pick-up device having a bio analysis signal generation unit for receiving a light excited by the biochip and outputting a bio analysis signal and a system and output controlling unit for outputting monitoring bio analysis information, the system and output controlling unit having a signal processing unit for processing and analyzing the bio-analysis signal corresponding to bio analysis information to generate the monitoring bio analysis information.

Claims

1. A diagnostic system having a biochip readout apparatus, comprising:

a biochip readout device including;

a biochip cartridge comprising;

(i) an optical disc comprising a first and a second substrate and a selective wavelength reflection film disposed on the first substrate, the first substrate having one or more substantially circular lands and grooves formed thereon, the second substrate having one or more depressed portions formed therein; and

(ii) at least one or more preformed biochips each comprising bio-cells spotted on the second substrate and forming an array having a substantially square or rectangular shape;

wherein the at least one or more biochips are removably installed in each of the depressed portions such that the at least one or more biochips cannot be separated from the optical disc when the optical disc is rotated or moved or the biochip is combined with another substrate thereon; and

the selective wavelength reflection film is disposed between the one or more biochips and the first substrate;

a disc rotation drive unit driven such that the biochip cartridge is rotated;

a system and output controlling unit for outputting monitoring bio analysis information, the system and output controlling unit having a signal processing unit for processing and analyzing the bio analysis signal corresponding to bio analysis information to generate the monitoring analysis information;

an optical pick-up device comprising;

a light receiving unit comprising one or more light sources and one or more light detectors wherein the one or more light detectors is a photomultiplier tube or photodiode;

wherein light from the one or more light sources is reflected by the reflective coating of the selective wavelength reflection film of the biochip cartridge and is detected by the one or more light detectors to obtain tracking and focusing signals for the optical pick-up device; and

light from the one or more light sources causes the one or more biochips to emit a fluorescent signal that is detected by the one or more light detectors;

a focusing/tracking controlling unit for controlling a focusing and tracking operation of the optical pick-up device using the tracking and focusing signals from the first light detector, so that the light from the one or more light sources tracks along the one or more lands and grooves of the biochip cartridge;

an objective lens driving unit for focusing the light from the one or more light sources;

a bio analysis signal generation unit for receiving the fluorescent signal emitted by the one or more biochips and outputting a bio analysis signal; and

an optical recording/reproducing unit for recording a recording bio analysis signal in a predetermined area of the biochip cartridge in response to a control signal of the system and output controlling unit and reproducing recorded biochip analysis information;

a mode selection unit for selecting one of a biochip readout mode and a general optical recording/reproducing mode; and

a diagnosis device for comparing the monitoring bio information for monitoring image signal from the biochip readout device with reference data and proving an analysis result generated based on a result of the comparing operation to a user, wherein the reference data for monitoring bio-information of the biochip are constructed in database format in the diagnosis device.

2. The biochip readout device as set forth in claim 1, wherein the bio analysis signal generation unit of the optical pick-up device scans the biochip cartridge with light in response to a control signal inputted from the system and output controlling unit, in case that the optical pick-up device has a single light source, and, at the same time, outputs a focusing/tracking controlling signal and the bio analysis signal caused by the light excited by the biochip.

3. The biochip readout device as set forth in claim 2, wherein the system and output controlling unit forms a matrix structure such that a cell revealing florescent dye is recognized as a letter of A and other cells are recognized as a letter of ˜

A, and generates monitoring bio analysis information based on the matrix structure.

4. The biochip readout device as set forth in claim 3,

wherein the bio analysis signal generation unit comprises:

an excited florescence filter for filtering an excited florescence wave of lights excited by the biochip; and

an excited florescent wave head for outputting the bio analysis signal based on detection of the filtered excited florescence wave in response to the control signal inputted from the system and output controlling unit.

5. The biochip readout system as set forth in claim 1, further comprising a communication device for transmitting an analysis processing request data together with the monitoring image signal thereto after inputting the monitoring image signal to analyze bio-matter from the biochip readout device and connecting communication lines thereto based on predetermined communication connection information.