Modified human interferon polypeptides and their uses

• US 8,119,603 B2
• Filed: 01/28/2005
• Issued: 02/21/2012
• Est. Priority Date: 02/02/2004
• Status: Expired due to Fees

• Current Assignee: Ambrx, Inc. (Johnson & Johnson)
• Original Assignee: Ambrx, Inc. (Johnson & Johnson)
• Inventors: Cho, Ho Sung, Daniel, Thomas O., Hays, Anna-Maria, Wilson, Troy E.
• Primary Examiner(s): Saoud, Christine J
• Assistant Examiner(s): Seharaseyon, Jegatheesan
• Application Number:
  US11/046,440
• Publication Number:
  US 20050220762A1
• Time in Patent Office: 2,580 Days
• Field of Search: None
• US Class Current: 514/21.2
• CPC Class Codes: A61K 38/21 Interferons [IFN]
• CPC Class Codes: A61K 38/27 Growth hormone [GH], i.e. somatotropin
• CPC Class Codes: A61K 47/60 the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 13/08 of the prostate
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 27/02 Ophthalmic agents
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/10 for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 35/02 specific for leukemia
• CPC Class Codes: A61P 37/02 Immunomodulators
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 5/00 Drugs for disorders of the endocrine system
• CPC Class Codes: A61P 5/06 of the anterior pituitary hormones, e.g. TSH, ACTH, FSH, LH, PRL, GH
• CPC Class Codes: A61P 5/10 of the posterior pituitary hormones, e.g. oxytocin, ADH
• CPC Class Codes: A61P 5/12 : for decreasing, blocking or antagonising the activity of the posterior pituitary hormones
• CPC Class Codes: A61P 7/06 : Antianaemics
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07H 21/04 : with deoxyribosyl as saccharide radical
• CPC Class Codes: C07K 14/475 : Growth factors; Growth regulators
• CPC Class Codes: C07K 14/52 : Cytokines; Lymphokines; Interferons
• CPC Class Codes: C07K 14/575 : Hormones derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin C07K14/665, e.g. corticotropin C07K14/695
• CPC Class Codes: C07K 14/61 : Growth hormone [GH], i.e. somatotropin
• CPC Class Codes: C08G 65/33396 : having oxygen in addition to nitrogen
• CPC Class Codes: G01N 2500/00 : Screening for compounds of potential therapeutic value
• CPC Class Codes: G01N 33/6875 : Nucleoproteins

Abstract

Modified human interferon polypeptides and uses thereof are provided.

Claims

1. An interferon (IFN) polypeptide comprising an amino acid sequence of SEQ ID NO:

24 wherein the IFN comprises one or more of linker or polymer wherein at least one of the linker or polymer is attached to a p-acetyl phenylalanine that is ribosomally incorporated into the polypeptide at position 107 of SEQ ID NO;

24 and wherein the IFN polypeptide has increased agonist activity when compared to an unmodified interferon.

2. The IFN polypeptide agonist of claim 1, wherein the water soluble polymer is a branched or multiarmed polymer.

3. The IFN polypeptide agonist of claim 2, wherein each branch of the branched polymer has a molecular weight of between about 1 kDa and about 100 kDa.

4. A composition comprising the IFN polypeptide agonist of claim 1 and a pharmaccutically acceptable carrier.

5. The IFN polypeptide agonist of claim 1 wherein said polymer is poly(ethylene glycol) molecule:

6. The IFN polypeptide agonist of claim 1, wherein the hIFN polypeptife comprises one said linker or a polymer.