Method and system for standardizing microscope instruments
First Claim
1. A method for standardizing a quantitative measurement of target sample data imaged by an optical system having an excitation light source, an optics portion, an image capture portion, and a data storage portion, cooperatively arranged for obtaining an image of the target sample, comprising:
- a. obtaining for the excitation light source, a light source correction factor;
b. applying the light source correction factor to the target sample data, thereby obtaining a target sample data standardized with regard to light intensity variability;
c. obtaining an optical system intrinsic factor;
d. applying the optical system intrinsic factor to the target sample data before determining the quantitative measurement of the standardized target sample data, the target sample data thereby standardized with regard to optical system intrinsic factors; and
e. determining a quantitative measure of the standardized target sample data.
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Accused Products
Abstract
Methods and apparatus for standardizing quantitative measurements from a microscope system. The process includes a calibration procedure whereby an image of a calibration slide is obtained through the optics of the microscope system. The calibration slide produces a standard response, which can be used to determine a machine intrinsic factor for the particular system. The machine intrinsic factor can be stored for later reference. In use, images are acquired of a target sample and of the excitation light source. The excitation light source sample is obtained using a calibration instrument configured to sample intensity. The calibration instrument has an associated correction factor to compensate its performance to a universally standardized calibration instrument. The machine intrinsic factor, sampled intensity, and calibration instrument correction factor are usable to compensate a quantitative measurement of the target sample in order to normalize the results for comparison with other microscope systems.
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Citations
12 Claims
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1. A method for standardizing a quantitative measurement of target sample data imaged by an optical system having an excitation light source, an optics portion, an image capture portion, and a data storage portion, cooperatively arranged for obtaining an image of the target sample, comprising:
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a. obtaining for the excitation light source, a light source correction factor; b. applying the light source correction factor to the target sample data, thereby obtaining a target sample data standardized with regard to light intensity variability; c. obtaining an optical system intrinsic factor; d. applying the optical system intrinsic factor to the target sample data before determining the quantitative measurement of the standardized target sample data, the target sample data thereby standardized with regard to optical system intrinsic factors; and e. determining a quantitative measure of the standardized target sample data. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A computer-usable medium having computer readable instructions stored thereon for execution by a processor to perform a method for standardizing a quantitative measurement of target sample data imaged by an optical system having an excitation light source, an optics portion, an image capture portion, and a data storage portion, cooperatively arranged for obtaining an image of the target sample, where the instructions comprise the steps of:
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a. obtaining for the excitation light source, a light source correction factor; b. applying the light source correction factor to the target sample data, thereby obtaining a target sample data standardized with regard to light intensity variability; c. obtaining an optical system intrinsic factor; d. applying the optical system intrinsic factor to the target sample data before determining the quantitative measurement of the standardized target sample data, the target sample data thereby standardized with regard to optical system intrinsic factors; and e. determining a quantitative measure of the standardized target sample data.
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12. A microscope system for obtaining a standardized quantitative measurement, of target sample data imaged by an optical system having an excitation light source, an optics portion, an image capture portion, and a data storage portion, cooperatively arranged for obtaining an image of the target sample, comprising:
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means for obtaining for the excitation light source, a light source correction factor; means for applying the light source correction factor to the target sample data, thereby obtaining a target sample data standardized with regard to light intensity variability; means for obtaining an optical system intrinsic factor; means for applying the optical system intrinsic factor to the target sample data before determining the quantitative measurement of the standardized target sample data, the target sample data thereby standardized with regard to optical system intrinsic factors; and means for determining a quantitative measure of the standardized target sample data.
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Specification