Control arrangement for microfluidic devices and related methods and systems

• US 8,123,192 B2
• Filed: 10/18/2007
• Issued: 02/28/2012
• Est. Priority Date: 10/18/2006
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Maltezos, George, Scherer, Axel
• Primary Examiner(s): FRISTOE JR, JOHN K
• Application Number:
  US11/874,211
• Publication Number:
  US 20080142157A1
• Time in Patent Office: 1,594 Days
• Field of Search: 251/129.17, 251/331
• US Class Current: 251/129.17
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: F16K 2099/0084 Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 99/0001 Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/0026 Valves using channel deformation
• CPC Class Codes: F16K 99/0034 Operating means specially adapted for microvalves
• CPC Class Codes: F16K 99/0046 using magnets
• CPC Class Codes: Y10T 156/1093 with covering of discrete laminae with additional lamina

Abstract

Communication between microchannels of a microfluidic circuit is controlled through a combination between a membrane and a pin-actuated valve arrangement. The deformable membrane contributes to the formation of the surface of the microchannels. Alternatively, or in addition, the valve arrangement is operated through combination of the membrane with a control fluid. Systems, devices and methods based on such communication are shown. Also shown are methods to manufacture a system having a fluidic circuit and a control arrangement.

Claims

1. A control arrangement for a fluidic circuit comprising one or more fluid channels, comprising:

a deformable membrane defining at least one surface of each of the one or more fluid channels, wherein the deformable membrane is formed with the one or more fluid channels in a monolithic microfluidic circuit;

an actuating arrangement comprising one or more actuating pins for deforming the deformable membrane, thus controlling flow in the one or more fluid channels by opening and/or closing the one or more fluid channels,

wherein the deformable membrane is made of the same material defining other surfaces of the one or more fluid channels.

2. The control arrangement of claim 1, wherein the deformable membrane defines a top surface or a bottom surface of the one or more fluid channels.

3. The control arrangement of claim 1, wherein the actuating pins are part of a solenoid actuator, the solenoid actuator comprising one or more electromagnetically driven solenoids moving the one or more actuating pins in contact or out of contact with the deformable membrane.

4. The control arrangement of claim 3, wherein the solenoid actuator is in a push-down configuration where the actuating pins are moved down to deform the deformable membrane or a push-up configuration where the actuating pins are moved up to deform the deformable membrane.

5. The control arrangement of claim 1, further comprising a control unit connected to the actuating arrangement.

6. The control arrangement of claim 1, wherein the one or more fluid channels have a rectangular or square profile, the deformable membrane defining at least one surface having a flat extension.

7. The control arrangement of claim 1, wherein the one or more fluid channels have a rounded surface, the deformable membrane defining at least one surface having a curved extension, the curved extension matching a curved surface of at least one pin deforming the deformable membrane.

8. The control arrangement of claim 1, wherein the deformable membrane is an integral part of the one or more fluid channels.

9. The control arrangement of claim 1, wherein the deformable membrane is separate from the one or more fluid channels.

10. The control arrangement of claim 1, wherein the deformable membrane is chemically bonded to the fluidic circuit.

11. A system with a fluidic circuit and a control arrangement for the fluidic circuit, the fluidic circuit including one or more fluid channels, the control arrangement comprising:

a deformable membrane defining at least one surface of each of the one or more fluid channels, wherein the deformable membrane is formed with the one or more fluid channels in a monolithic microfluidic circuit; and

an actuating arrangement comprising one or more actuating pins for deforming the deformable membrane, thus controlling flow in the one or more fluid channels by opening and/or closing the one or more fluid channels, the system comprising;

a reinforcing layer keeping the fluidic circuit and the deformable membrane together,

wherein the reinforcing layer comprises holes to allow passage of the one or more actuating pins.

12. The system of claim 11, wherein the deformable membrane substantially covers an entire surface of the fluidic circuit.

13. The system of claim 11, wherein the fluidic circuit is a disposable fluidic circuit and the control arrangement is a multi-use control arrangement.

14. A control arrangement for a fluidic circuit comprising one or more fluid channels, comprising:

a deformable membrane defining at least one surface of each of the one or more fluid channels, wherein the deformable membrane is formed with the one or more fluid channels in a monolithic microfluidic circuit;

an actuating arrangement comprising one or more actuating pins for deforming the deformable membrane, thus controlling flow in the one or more fluid channels by opening and/or closing the one or more fluid channels;

a first storage compartment and a second storage compartment, the first storage compartment fluidly in communication with the second storage compartment; and

one or more fingers and clamps, configured to deform the deformable membrane to seal the one or more fluid channels, wherein the deformed deformable membrane maintains fluidic separation between the first storage compartment and the second storage compartment.

15. The control arrangement of claim 14, wherein the one or more fingers and clamps are configured to exert a spring-like force on the deformable membrane.

16. The control arrangement of claim 14, wherein the one or more fingers and clamps are electromechanically actuated.

17. The control arrangement of claim 14, wherein the one or more fluid channels are normally sealed with the one or more fingers and clamps, and the one or more fluid channels are unsealed when actuated.

18. The control arrangement of claim 14, wherein the first storage compartment is adapted to store a first reagent and the second storage compartment is adapted to store a second reagent, such that when the one or more fingers and clamps are actuated to unseal the one or more fluid channels, the first reagent and the second reagent are combined.

19. The control arrangement of claim 18, wherein the first reagent and the second reagent are selected from the group consisting:

a lyophilized reagent and a wet reagent respectively, a wet reagent and a lyophilized reagent respectively, a lyophilized reagent and a lyophilized reagent respectively, and a wet reagent and a wet reagent respectively.