Apparatus and methods for microfluidic applications
First Claim
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1. A microfluidic processing apparatus for use in gel electrophoresis, the apparatus comprising:
- a planar supporting layer comprising a pattern of conductive tracks; and
a single layer of deformable polymer film comprising a three-dimensional microfluidic processing area that is formed from and extends out of a plane of said single layer of deformable polymer film, said three-dimensional microfluidic processing area comprising at least one cavity, at least one connecting channel and at least one pierceable access zone;
wherein said microfluidic processing area contains a gel, andwherein said pattern of conductive tracks are configured to permit application of an electrical field through said gel.
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Abstract
Non-rigid tape apparatus and fabrication methods for microfluidic processing applications such as gel electrophoresis are provided, where microfluidic processing is performed on selected areas. Parts of the tape are formed by high pressure plastic film forming. Membranes and other structures are self sealing during and after penetration by pipettes and electrical probes. Rigid exoskeleton elements protect the non-rigid parts during processing and facilitate transport of the tape.
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Citations
21 Claims
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1. A microfluidic processing apparatus for use in gel electrophoresis, the apparatus comprising:
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a planar supporting layer comprising a pattern of conductive tracks; and a single layer of deformable polymer film comprising a three-dimensional microfluidic processing area that is formed from and extends out of a plane of said single layer of deformable polymer film, said three-dimensional microfluidic processing area comprising at least one cavity, at least one connecting channel and at least one pierceable access zone; wherein said microfluidic processing area contains a gel, and wherein said pattern of conductive tracks are configured to permit application of an electrical field through said gel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification