Microfluidic device and method of using same
First Claim
1. A microfluidic device comprising:
- a. at least one first channel for introducing a first fluid into the device;
b. a particle retention structure spaced-apart from the first channel for retaining a particle therein, wherein said particle retention structure is generally V-shaped in a top plan view, the particle retention structure having opposed wall portions and a central wall portion disposed between and joining the opposed wall portions, wherein the particle retention structure is located generally opposite the first channel, and wherein the opposed and central wall portions have sloped side walls extending from an upper portion thereof to a lower portion of said microfluidic device; and
c. one or more fluid ports disposed between the first channel and the particle retention structure for introducing a second fluid into the microfluidic device, and for allowing one or more of the first and second fluids to flow out of the microfluidic device.
1 Assignment
0 Petitions
Accused Products
Abstract
The invention relates to a microfluidic device comprising one or more fluid channels, one or more fluid ports, and a V-shaped particle retention structure. The fluid channel is generally opposite the particle retention structure, fluid ports are located between the fluid channel and the particle retention structure, and the particle retention structure has sloped side walls. Fluid, including reagents, can be delivered to the microfluidic device through the one or more fluid channels or the fluid ports. The invention also relates to methods of using the microfluidic device to monitor, observe, measure, or record a biological parameter of a particle, to separate a single particle from a group of particles, to culture a cell, to treat a particle, and to move a particle back and forth in the device.
9 Citations
39 Claims
-
1. A microfluidic device comprising:
-
a. at least one first channel for introducing a first fluid into the device; b. a particle retention structure spaced-apart from the first channel for retaining a particle therein, wherein said particle retention structure is generally V-shaped in a top plan view, the particle retention structure having opposed wall portions and a central wall portion disposed between and joining the opposed wall portions, wherein the particle retention structure is located generally opposite the first channel, and wherein the opposed and central wall portions have sloped side walls extending from an upper portion thereof to a lower portion of said microfluidic device; and c. one or more fluid ports disposed between the first channel and the particle retention structure for introducing a second fluid into the microfluidic device, and for allowing one or more of the first and second fluids to flow out of the microfluidic device. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
-
-
22. A microfluidic device comprising:
-
a. one or more first channels for introducing a first fluid into the device; b. a particle retention structure spaced-apart from the first channel for retaining a particle therein, wherein said particle retention structure is generally V-shaped in a top plan view, the particle retention structure having opposed wall portions and a central wall portion disposed between and joining the opposed wall portions, wherein the particle retention structure is located generally opposite the first channel, and wherein the opposed and central wall portions have sloped side walls extending from an upper portion thereof to a lower portion of said microfluidic device; and c. fluid ports disposed between the first channel and the particle retention structure for introducing a second fluid into the microfluidic device, and for allowing one or more of the first and second fluids to flow out of the microfluidic device.
-
Specification