Compositions and assays to detect influenza virus A and B nucleic acids
First Claim
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1. A composition comprising at least two isolated nucleic acid oligomers specific for influenza virus B consisting of SEQ ID NO:
- 39 and SEQ ID NO;
44, or completely complementary sequences to SEQ ID NO;
39 and SEQ ID NO;
44.
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Abstract
Methods for detecting influenza virus A and influenza virus B nucleic acids in biological samples by using in vitro amplification and detection are disclosed. Compositions that are target-specific nucleic acid sequences and kits comprising target-specific nucleic acid oligomers for amplifying in vitro influenza virus A or influenza virus B nucleic acid and detecting amplified nucleic acid sequences are disclosed.
20 Citations
30 Claims
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1. A composition comprising at least two isolated nucleic acid oligomers specific for influenza virus B consisting of SEQ ID NO:
- 39 and SEQ ID NO;
44, or completely complementary sequences to SEQ ID NO;
39 and SEQ ID NO;
44. - View Dependent Claims (2, 3, 4)
- 39 and SEQ ID NO;
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5. A method of detecting nucleic acid of influenza virus B in a sample, comprising the steps of:
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providing a sample containing influenza virus B nucleic acid; amplifying a target sequence in the influenza virus B nucleic acid contained in the sample by using a nucleic acid polymerase and at least two isolated nucleic acid oligomers consisting of the sequences of SEQ ID Nos. 39 and 44, or the complete complements of the sequences consisting of SEQ ID Nos. 39 and 44 in vitro to produce an amplified product under substantially isothermal conditions, and detecting the amplified product, thereby detecting the presence of influenza virus B in the sample. - View Dependent Claims (6, 7, 8, 9, 10, 11)
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12. A composition comprising at least two isolated nucleic acid oligomers specific for influenza virus B wherein one oligomer has a target hybridizing sequence that is from 19 to 21 nucleotides in length and contains a sequences consisting essentially of SEQ ID NO:
- 42, the complement and/or RNA equivalent thereof and, optionally, contains at least one 2′
methoxy RNA group or at least one LNA; and
wherein a second oligomer has a sequence that is from 19 to 22 nucleotides in length and contains a sequence consisting essentially of SEQ ID NO;
44, the complement and/or RNA equivalent thereof and, optionally, contains at least one 2′
methoxy RNA group or at least one LNA. - View Dependent Claims (13, 14, 15, 16, 17, 18, 30)
- 42, the complement and/or RNA equivalent thereof and, optionally, contains at least one 2′
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19. A method of detecting nucleic acid of influenza virus B in a sample, comprising the steps of:
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providing a sample containing influenza virus B nucleic acid; amplifying a target sequence in the influenza virus B nucleic acid contained in the sample by using a nucleic acid polymerase and at least two isolated nucleic acid amplification oligomers, wherein the at least two nucleic acid amplification oligomers includes an amplification oligomer 19 to 21 nucleotides in length consisting essentially of the nucleotide target hybridizing sequences of SEQ ID NO;
42, the complement and/or RNA equivalent thereof and, optionally, contains at least one 2′
methoxy RNA group or at least one LNA, and wherein the at least two nucleic acid amplification oligomers includes an amplification oligomer 19 to 22 nucleotides in length consisting essentially of the nucleotide sequences of SEQ ID NO;
44, the complement and/or RNA equivalent thereof and, optionally, contains at least one 2′
methoxy RNA group or at least one LNA, in vitro to produce an amplified product under substantially isothermal conditions, anddetecting the amplified product, thereby detecting the presence of influenza virus B in the sample. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification