Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermentors
First Claim
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1. A process for producing lipids containing polyunsaturated fatty acids from microorganisms of the order Thraustochytriales by conducting a fermentation of the microorganisms in a medium comprising a carbon source, the process comprising:
- a) providing the fermentation medium comprising the carbon source;
b) adding the microorganisms to the fermentation medium;
c) adding an additional amount of the carbon source to the fermentation medium in a fed-batch process to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least 4% of saturation;
d) allowing the dissolved oxygen content of the fermentation medium to decrease to less than about 3% of saturation; and
e) recovering at least some of the lipids from the microorganisms;
wherein the lipids are at least 20% by weight of the dry cell weight of the biomass.
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Abstract
The present invention provides a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids. The present invention also provides a process for producing eukaryotic microbial lipids.
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Citations
6 Claims
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1. A process for producing lipids containing polyunsaturated fatty acids from microorganisms of the order Thraustochytriales by conducting a fermentation of the microorganisms in a medium comprising a carbon source, the process comprising:
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a) providing the fermentation medium comprising the carbon source; b) adding the microorganisms to the fermentation medium; c) adding an additional amount of the carbon source to the fermentation medium in a fed-batch process to increase the biomass density of the fermentation medium when the dissolved oxygen content of the fermentation medium is at least 4% of saturation; d) allowing the dissolved oxygen content of the fermentation medium to decrease to less than about 3% of saturation; and e) recovering at least some of the lipids from the microorganisms; wherein the lipids are at least 20% by weight of the dry cell weight of the biomass. - View Dependent Claims (2, 3, 4, 5, 6)
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