Thermocycler and sample port

• US 8,124,413 B2
• Filed: 02/02/2007
• Issued: 02/28/2012
• Est. Priority Date: 02/02/2006
• Status: Expired due to Fees

• Current Assignee: QIAGEN Instruments AG (Qiagen NV)
• Original Assignee: Corbett Life Science (Qiagen NV)
• Inventors: Stanley, Keith, Corbett, John
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): HAMMOND, CHARLES
• Application Number:
  US12/162,942
• Publication Number:
  US 20090220966A1
• Time in Patent Office: 1,852 Days
• Field of Search: 422/82, 436 52- 53
• US Class Current: 436/53
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0838 Capillaries
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: B01L 7/54 using spatial temperature gradients
• CPC Class Codes: Y10T 137/0318 Processes
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.

Claims

1. A method for introducing a volume of a liquid sample into a fluid carrier stream flowing through a continuous flow tube having an outlet and a common inlet into which said carrier stream and said liquid sample are both introduced, said method comprising the steps of:

providing a sample port comprising a reservoir for continuously supplying said common inlet with said fluid carrier, and an inlet for supplying said reservoir with fluid carrier, said reservoir adapted to maintain a substantially constant level of fluid carrier above said common inlet and fluidly engageable with said common inlet of said continuous flow tube;

fluidly engaging said inlet of said continuous flow tube with said reservoir; and

introducing said fluid carrier into said reservoir and introducing said liquid sample into said fluid carrier, wherein said fluid carrier is chosen such that its properties are sufficient to maintain the physical shape of the liquid sample and wherein said fluid carrier stream and said liquid sample are drawn through said continuous flow tube when said reservoir is at substantially atmospheric pressure.

2. A method according to claim 1, wherein the liquid sample is an aqueous sample.

3. A method according to claim 1, wherein the carrier fluid is a hydrophobic liquid.

4. A method according to claim 3, wherein the hydrophobic liquid is an oil.

5. A method according to claim 4, wherein the oil is a silicone oil or a silicone-based oil.

6. A method according to claim 1, wherein said reservoir is open to atmospheric pressure during use.

7. A method for introducing a volume of a liquid sample into a fluid carrier stream flowing through a continuous flow tube having an outlet and a common inlet into which said carrier stream and said liquid sample are both introduced, said method comprising the steps of:

providing a sample port comprising a reservoir for continuously supplying said common inlet with said fluid carrier, and an inlet for supplying said reservoir with fluid carrier, said reservoir adapted to maintain a substantially constant level of fluid carrier above said common inlet and fluidly engageable with said common inlet of said continuous flow tube;

fluidly engaging said inlet of said continuous flow tube with said reservoir;

introducing said fluid carrier into said reservoir;

immersing a liquid sample dispenser into said fluid carrier contained in said reservoir; and

dispensing said liquid sample adjacent said inlet and manoeuvring said dispensed liquid sample with said liquid sample dispenser such that said dispensed liquid sample is introduced into said inlet and drawn through said continuous flow tube, wherein said fluid carrier is chosen such that its properties are sufficient to maintain the physical shape of the liquid sample and wherein said fluid carrier stream and said liquid sample are drawn through said continuous flow tube when said reservoir is at substantially atmospheric pressure.

8. A method according to claim 7, wherein said reservoir is open to atmospheric pressure during use.

9. A method according to claim 7, wherein the liquid sample is an aqueous sample.

10. A method according to claim 7, wherein the carrier fluid is a hydrophobic liquid.

11. A method according to claim 10, wherein the hydrophobic liquid is an oil.

12. A method according to claim 11, wherein the oil is a silicone oil or a silicone-based oil.