Methods and kits using extended rhodamine dyes
First Claim
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1. A kit for fragment analysis comprising one or more nucleoside or nucleotide, a chain-terminating nucleotide analog and a primer, wherein one or more of the nucleoside or nucleotide is a labeled nucleic acid compound comprising the structure:
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Abstract
Extended rhodamine compounds exhibiting favorable fluorescence characteristics having the structure
are disclosed. In addition, novel intermediates for synthesis of these dyes are disclosed, such intermediates having the structure
In addition, methods of making and using the dyes as fluorescent labels are disclosed.
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Citations
4 Claims
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1. A kit for fragment analysis comprising one or more nucleoside or nucleotide, a chain-terminating nucleotide analog and a primer, wherein one or more of the nucleoside or nucleotide is a labeled nucleic acid compound comprising the structure:
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2. A kit for fragment analysis comprising one or more nucleotide triphosphates, a chain-terminating nucleotide analog and a primer, wherein one or more of the nucleotide triphosphates is a labeled nucleic acid compound comprising the structure:
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3. A kit for fragment analysis comprising one or more nucleotide triphosphates, a chain-terminating nucleotide analog and a primer, wherein said chain-terminating nucleotide analog is a labeled nucleic acid compound comprising the structure:
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4. A kit for fragment analysis comprising one or more nucleotide triphosphates, a chain-terminating nucleotide analog and a primer, wherein said chain-terminating nucleotide analog is a labeled nucleic acid compound comprising the structure:
Specification