Biosensor

• US 8,128,795 B2
• Filed: 03/28/2006
• Issued: 03/06/2012
• Est. Priority Date: 03/29/2005
• Status: Active Grant

• Current Assignee: Citizen Watch Co Limited (Citizen Electronics Company Limited)
• Original Assignee: Citizen Holdings Co., Ltd.
• Inventors: Egawa, Shunji
• Primary Examiner(s): Noguerola, Alex
• Assistant Examiner(s): Kaur, Gurpreet
• Application Number:
  US11/390,476
• Publication Number:
  US 20060243590A1
• Time in Patent Office: 2,170 Days
• Field of Search: 20440301-40315, 204/412, 422/506, 422/504, 422/533, 422/72, 137/625.4, 137/833
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52

Abstract

The present invention is directed to a biosensor being provided with a suction cavity which sucks a certain amount of sample by means of capillary phenomenon, the biosensor including a flow channel to connect the suction cavity and an analytical cavity furnished with a reagent, wherein, the flow channel has a narrowed section provided with a gap formed by narrowing a flow passage area. The narrowed section has a function to retain the sample in the suction cavity when the sample is sucked therein, and a function to circulate the sample retained in the suction cavity into the analytical cavity through the gap, when a centrifugal force is applied from the outside. The two functions held by the narrowed section enables a simple collection of blood sample, and further enables a simple transfer of the blood sample to the analytical area by applying the centrifugal force to the blood sample, as well as facilitating a component extraction.

Claims

1. A biosensor being provided with a suction cavity which sucks a certain amount of sample by means of capillary phenomenon, said biosensor comprising:

a flow channel to connect said suction cavity and an analytical cavity furnished with a reagent, and

a wall portion formed by projecting at least any one of an upper surface, a lower surface, and side walls of said flow channel, said wall portion provided on a part of said flow channel to form a narrowed section, said narrowed section is provided with a gap formed by narrowing a flow passage area due to said wall portion provided on said part of the flow channel,

said sample is held back by the wall portion of said narrowed section so that said sample is retained in said suction cavity when the sample is sucked into said suction cavity, and allows the sample accumulated in said suction cavity to circulate into said analytical cavity through said gap, when a centrifugal force is applied from the outside, and

said analytical cavity is not provided with an air vent, said analytical cavity vents air displaced by the sample from said suction cavity by way of the gap in the flow channel which communicates with an outlet for venting air provided in said suction cavity,

wherein said narrowed section comprises;

a first portion where a flow channel area is gradually decreased on the suction cavity side, and

a second portion where the flow channel area stays narrow for a predetermined distance between said suction cavity side and said flow channel side, and wherein,

said narrow area increases in the rear of said second portion, from said flow channel area having been narrowed to an original area of said flow channel.

2. The biosensor according to claim 1, wherein,

said analytical cavity comprises an enzyme reaction layer for use in electrochemical measurement.

3. The biosensor according to claim 2, wherein,

a plurality of said enzyme reaction layers are separately arranged along a direction to which the centrifugal force is applied, and said enzyme reaction layers are respectively associated with components of the sample being centrifugally separated.

4. The biosensor according to at least any one of claims 1 to 3, wherein, said suction cavity comprises:

two openings placed in parallel, perpendicularly to the direction to which the centrifugal force is applied, and

a joint which establishes connection with said flow channel, and,

at said joint, a line from each of said two openings to said joint crosses the direction to which the centrifugal force is applied, in a positional relationship to form an obtuse angle, and the sample in said suction cavity receives a force directing to said flow channel by the centrifugal force.

5. The biosensor according to at least any one of claims 1 to 3, wherein,

said suction cavity comprises a reagent for pretreating said sample.

6. The biosensor according to at least any one of claims 1 to 3, wherein, said suction cavity, flow channel, and analytical cavity are formed by bonding two plate-like members with surfaces opposed to each other, one or both of the surfaces having concave-convex parts.

7. The biosensor according to claim 6, wherein,

an electrode substrate is placed between said two plate-like members, the electrode substrate having an electrode being exposed in said analytical cavity.

8. The biosensor according to claim 7, wherein,

at least one of said plate-like members comprises a window part which electrically connects a connector intended for external connection with an external circuit, said connector being electrically connected with said electrode on the electrode substrate.

9. The biosensor according to claim 8, wherein,

said plate-like member comprises either of an O-ring and a packaging member which surrounds said window part.

10. The biosensor according to claim 9, wherein,

said plate-like member comprises either of an O-ring and a packaging member which surrounds a plurality of said window parts as a whole.

11. The biosensor according to claim 9, wherein, said plate-like member comprises either of a plurality of O-rings and a plurality of packaging members which respectively surround a plurality of said window parts individually.

12. The biosensor according to claim 8, wherein,

said window part intended for the external connection is placed at a position closer to the center of the centrifugal force, which is at least closer than said analytical cavity, in the direction to which the centrifugal force is applied.

13. The biosensor according to at least any one of claims 1 to 3, wherein,

ultrasonic welding is applied to an outer circumferential part of said suction cavity, flow channel, and analytical cavity.

14. The biosensor according to at least any one of claims 1 to 3, wherein,

an outermost circumferential part of said plate-like member is subjected to the ultrasonic welding.

15. The biosensor according to at least any one of claims 1 to 3, wherein,

hydrophilic coating is applied on a wall surface of said suction cavity.

16. The biosensor according to at least any one of claims 1 to 3, wherein,

said hydrophilic coating is formed by applying surfactant.

17. The biosensor according to at least any one of claims 1 to 3, wherein,

said narrowed section is hydrophobic.

18. The biosensor according to claim 17, wherein,

said narrowed section is made of a hydrophobic synthetic resin.

19. The biosensor according to claim 17,

a water-repellent treatment is applied on said narrowed section.