Analyzing article, analyzer and method of analyzing a sample using the analyzing article, and a method of forming an opening in the analyzing article

• US 8,128,889 B2
• Filed: 04/28/2003
• Issued: 03/06/2012
• Est. Priority Date: 04/30/2002
• Status: Active Grant

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Fujimoto, Koji, Kitamura, Shigeru, Hori, Masaki
• Primary Examiner(s): Wright, P. Kathryn
• Application Number:
  US10/514,010
• Publication Number:
  US 20050282290A1
• Time in Patent Office: 3,235 Days
• Field of Search: None
• US Class Current: 422/417
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention relates to a technique for moving a mobile component such as a sample and a reagent, in an analyzing article. A method of analyzing a sample according to the present invention includes a first movement step of moving a mobile component from a fluid entrance port (61) to a first goal (511) in a passage (51), and a second movement step of moving the mobile component from the first goal (511) to a second goal (512) in the passage (51), in an analyzing article (Y1). The analyzing article (Y1) further includes a branch passage which branches from the first goal (511), and a first opening for releasing gas from within the branch passage. The first movement step is carried out by establishing communication between inside and outside of the branch passage via the first opening. The second movement step is carried out by providing a second opening downstream from the second goal (512) with respect to a flow direction of the mobile component, thereby establishing communication between inside and outside of the passage.

Claims

1. An analyzer for analysis of a sample comprising:

an analyzing article including a plurality of individual passages each for moving a mobile component from a fluid reservoir via a first goal to a second goal, a common passage extending from each individual passage to another for connecting the plurality of individual passages downstream from the second goal of each individual passage in a flow direction of the mobile component, and an analyzer unit for analyzing the mobile component of the sample,

the analyzing article preventing the mobile component from moving beyond the first goal in the passage while allowing the mobile component to move beyond the first goal to the second goal in each individual passage by opening a common gas release port communicating with the common passage, the common gas releasing port being closed by a common closer, wherein the analyzer further comprises an common opener for opening the common gas release port at the downstream location, wherein each individual passage extends horizontally throughout an entire path from the fluid reservoir to the common passage, and wherein the analyzing article comprises a substrate formed with the plurality of individual passages, and a cover attached to the substrate for covering the plurality of individual passages, the common passage being formed in the cover above the plurality of individual passages and configured to prevent the mobile component of the sample from flowing into the common passage.

2. The analyzer according to claim 1, wherein the analyzing article allows communication between inside of each individual passage and outside,

the common opener opening the common gas releasing port by forming an opening in the common closer.

3. The analyzer according to claim 2, wherein the common closer is provided by a sheet member,

the common opener including a cutter for cutting the sheet member.

4. The analyzer according to claim 2, wherein the common closer is provided by a plug member inserted into the common gas releasing port,

the common opener including a puller for pulling out the plug member.

5. The analyzer according to claim 1, wherein the common opener applies energy to the analyzing article without contacting the analyzing article for melting or deforming at least part of the analyzing article.

6. The analyzer according to claim 1,

wherein the analyzing article further includes a branch passage branched from a branching region in each individual passage to provide the first goal, the branch passage communicating with outside for preventing the mobile component from moving in each individual passage beyond the branching region.

7. The analyzer according to claim 6, wherein the common closer includes a movable closing element for removable insertion into the common gas release port.