Method and device for image-based biological data quantification

• US 8,131,477 B2
• Filed: 08/08/2006
• Issued: 03/06/2012
• Est. Priority Date: 11/16/2005
• Status: Active Grant

• Current Assignee: Thales DIS France SAS (Thales SA)
• Original Assignee: 3M Cogent Incorporated (Thales SA)
• Inventors: Li, Songtao, Hsieh, Ming, Tang, Xian, Wang, Ankuo
• Primary Examiner(s): Clow, Lori A
• Application Number:
  US11/501,377
• Publication Number:
  US 20070112525A1
• Time in Patent Office: 2,037 Days
• Field of Search: None
• US Class Current: 702/19
• CPC Class Codes: G06T 2207/30072 Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 Biomedical image inspection

Abstract

A device and method for determining a concentration of a biological target including capturing an image of a ticket that contains the biological target; extracting a region of interest from the captured image; detecting a target from the extracted region of interest; computing intensity of the target in the region of interest; and determining the concentration of the biological target according to the computed intensity.

Claims

1. A method for determining a concentration of a biological target, the method comprising:

capturing an image of a ticket including the biological target;

extracting a region of interest from the captured image;

detecting a target from the extracted region of interest comprising a multi-level target detection based on a concentration of the target, the multi-level target detection including;

detecting a target with a high concentration by applying an edge detection and circular contour analysis to extract the target with a high concentration;

detecting a target with a medium concentration by analyzing a local grayscale distribution to separate a target area with circular shape background; and

detecting a target with a low concentration by extracting partial circular shape features from the background;

electronically computing intensity of the target in the region of interest; and

determining the concentration of the biological target according to the computed intensity.

2. The method of claim 1, wherein electronically computing intensity comprises electronically computing the intensity of the target in the region of interest according to image grayscale differences of image grayscale values between the target and a background area.

3. The method of claim 2, wherein electronically computing intensity comprises:

computing an average grayscale value for the target area and the background area;

computing an average grayscale value for the target area;

computing an average grayscale value for the background area; and

electronically computing the target intensity responsive to the average grayscale for the target area and the average grayscale for the background area.

4. The method of claim 1, wherein determining the concentration comprises selecting pre-determined data related to intensity and concentration of the target, and electronically determining the concentration of the target according to the pre-determined data.

5. The method of claim 4, wherein the pre-determined data is a look-up table stored in a memory.

6. The method of claim 1, wherein detecting a target comprises:

electronically computing a gradient magnitude value for each pixel in the region of interest;

comparing the computed gradient magnitude value to a pre-determined threshold value; and

classifying a pixel as being within the region of interest, if the computed gradient magnitude value of the pixel is larger than the pre-determined threshold value.

7. The method of claim 1, further comprising displaying the concentration of the biological target on a display.

14. The device of claim 1, further comprising a display panel for displaying the concentration of the biological target.

8. A portable device for determining a concentration of a biological target comprising:

an optical module for capturing an image of a ticket including the biological target;

a sensor for converting the captured image to digital data; and

a processor for processing the digital data, the processor comprising;

an image calibration module for checking the ticket validity from the captured image,

a target detection module for detecting a target from the extracted region of interest using a multi-level target detection based on a concentration of the target, wherein the target detection module is configured for;

detecting a target with a high concentration by applying an edge detection and circular contour analysis to extract the target with a high concentration;

detecting a target with a medium concentration by analyzing a local grayscale distribution to separate a target area with circular shape background; and

detecting a target with a low concentration by extracting partial circular shape features from the background, and

a target quantification module for computing intensity of the target in the region of interest and determining the concentration of the biological target according to the computed intensity.

9. The device of claim 8, wherein the target quantification module computes the intensity of the target in the region of interest according to image grayscale differences of image grayscale values between the target and a background area.

10. The device of claim 9, wherein the target quantification module computes an average grayscale value for the target area and the background area, computes an average grayscale value for the target area, computes an average grayscale value for the background area, and computes the target intensity responsive to the average grayscale for the target area and the average grayscale for the background area.

11. The device of claim 8, wherein the target quantification module selects pre-determined data related to intensity and concentration of the target and determines the concentration of the target according to the pre-determined data.

12. The device of claim 11, wherein the pre-determined data is a look-up table stored in a memory.

13. The device of claim 8, wherein the target detection module computes a gradient magnitude value for each pixel in the region of interest, compares the computed gradient magnitude value to a pre-determined threshold value, and classifies a pixel as being within the region of interest, if the computed gradient magnitude value of the pixel is larger than the pre-determined threshold value.

15. A device for determining a concentration of a biological target comprising:

means for capturing an image of a ticket including the biological target;

means for extracting a region of interest from the captured image;

means for detecting a target from the extracted region of interest comprising a multi-level target detection based on a concentration of the target, the multi-level target detection including;

means for detecting a target with a high concentration by applying an edge detection and circular contour analysis to extract the target with a high concentration

means for detecting a target with a medium concentration by analyzing a local grayscale distribution to separate a target area with circular shape background; and

means for detecting a target with a low concentration by extracting partial circular shape features from the background;

means for computing intensity of the target in the region of interest; and

means for determining the concentration of the biological target according to the computed intensity.

16. The device of claim 15, wherein means for computing intensity of the target comprises means for computing the intensity of the target according to image grayscale differences of image grayscale values between the target and a background area.

17. The device of claim 16, wherein means for computing intensity comprises:

means for computing an average grayscale value for the target area and the background area;

means for computing an average grayscale value for the target area;

means for computing an average grayscale value for the background area; and

means for computing the target intensity responsive to the average grayscale for the target area and the average grayscale for the background area.

18. The device of claim 15, wherein means for determining the concentration comprises means for selecting pre-determined data related to intensity and concentration of the target, and means for determining the concentration of the target according to the pre-determined data.

19. The device of claim 18, wherein the pre-determined data is a look-up table stored in a memory.

20. The device of claim 15, wherein means for detecting a target comprises:

means for computing a gradient magnitude value for each pixel in the region of interest;

means for comparing the computed gradient magnitude value to a pre-determined threshold value; and

means for classifying a pixel as being within the region of interest, if the computed gradient magnitude value of the pixel is larger than the pre-determined threshold value.