Method for magnetic separation of red blood cells from a patient sample

• US 8,137,903 B2
• Filed: 12/16/2008
• Issued: 03/20/2012
• Est. Priority Date: 12/20/2007
• Status: Expired due to Fees

• Current Assignee: Cytyc Corporation (Hologic Incorporated)
• Original Assignee: Cytyc Corporation (Hologic Incorporated)
• Inventors: Kaufman, Howie, Burg, Lawrence
• Primary Examiner(s): Saucier, Sandra
• Application Number:
  US12/335,748
• Publication Number:
  US 20110104778A1
• Time in Patent Office: 1,190 Days
• Field of Search: None
• US Class Current: 435/2
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/80 involving blood groups or blood types or red blood cells white blood cells G01N33/56972

Abstract

A method for separating components from a patient sample is provided. In particular, the present invention provides a method for the separation of red blood cells or red blood cell components from a patient sample by the use of magnetic beads.

Claims

1. A method of separating red blood cells or red blood cell fragments from other components of a biological sample in a specimen container the method comprising the steps of:

i.) adding to a specimen container a biological sample containing red blood cells or red blood cell fragments, which specimen container comprises an inner sleeve and an outer sleeve defining an internal compartment therebetween containing magnetic beads, said magnetic beads comprising a binder capable of selectively binding to red blood cells or red blood cell fragments;

ii.) removing said inner sleeve from said outer sleeve thus allowing said magnetic beads to come into fluid contact with said biological sample;

iii.) allowing the magnetic beads to selectively bind to the red blood cells or red blood cell fragments in the specimen container;

iv.) positioning a magnetic field in close proximity to the magnetic beads in the specimen container such that the beads are manipulated to the inner wall of the outer sleeve by the magnetic field; and

v.) reinserting said inner sleeve into said outer sleeve such that the red blood cells or red blood cell fragments separate from other cellular components of the patient sample in the specimen container.

2. The method of claim 1 wherein the biological sample comprises cervical cells.

3. The method of claim 1 wherein the biological sample is suspended in a cell preservative solution.

4. The method of claim 1 wherein the magnetic beads are immobilized by the magnetic field to the inner surface of the specimen container within an integrated compartment.

5. The method of claim 1 wherein red blood cells or red blood cell fragments are manipulated from other cell components in the biological sample by removing the magnetic beads from the specimen container by the magnetic field after the magnetic beads have selectively bound to the red blood cells or the red blood cell fragments.

6. The method of claim 1 wherein the magnetic beads are paramagnetic.

7. A method of separating red blood cells or red blood cell fragments from other cellular components in specimen container containing cells of a patient specimen, the method comprising the steps of:

i.) adding to a specimen container a patient specimen comprising red blood cells or red blood cell fragments, which specimen container comprises as inner sleeve and an outer sleeve defining an internal compartment therebetween containing magnetic beads, said magnetic beads comprising a binder capable of selectively binding to red blood cells or red blood cell fragments;

ii.) removing said inner sleeve from said outer sleeve thus allowing said magnetic beads to come into fluid contact with said biological sample;

iii.) allowing the magnetic beads to selectively bind to hemoglobin in the red blood cells or red blood cell fragments;

iv.) positioning a magnetic field in close proximity to the magnetic beads in the specimen container such that the magnetic beads are manipulated towards the magnet field;

whereby the manipulation of the magnetic beads towards the magnetic field results in the red blood cells or red blood cell fragments separating from other cellular components of the patient specimen in the specimen container.

8. The method of claim 7 wherein the patient sample comprises cervical cells.

9. The method of claim 7 wherein the patient sample is suspended in a cell preservative solution.

10. The method of claim 7 wherein the magnetic beads are immobilized by the magnetic field to the inner surface of the specimen container or within an integrated compartment.

11. The method of claim 7 wherein red blood cells or red blood cell fragments are separated from other cell components in the patient sample by removing the magnetic beads from the specimen container by the magnetic field after the magnetic beads have selectively bound to the red blood cells or the red blood cell fragments.

12. The method of claim 7 wherein the magnetic beads are paramagnetic.

13. A method of separating red blood cells or red blood cell fragments from other cellular components in specimen container containing cervical cells suspended in a preservative fluid, the method comprising the steps of:

i.) adding to a specimen container a patient specimen comprising red blood cells or red blood cell fragments, which specimen container comprises as inner sleeve and an outer sleeve defining an internal compartment therebetween containing magnetic beads, said magnetic beads comprising aptamers specific to hemoglobin bound thereto;

ii.) removing said inner sleeve from said outer sleeve thus allowing said magnetic beads to come into fluid contact with said biological sample;

iii.) allowing the magnetic beads to selectively bind to hemoglobin in the red blood cells or red blood cell fragments;

iv.) positioning a magnetic field in close proximity to the magnetic beads in the specimen container such that the magnetic beads are manipulated towards the magnet field;

whereby the manipulation of the magnetic beads towards the magnetic field results in the red blood cells or red blood cell fragments separating from other cellular components of the patient specimen in the specimen container.

14. The method of claim 13 wherein the magnetic beads are immobilized by the magnet to the inner surface of the specimen container or within an integrated compartment.

15. The method of claim 13 wherein red blood cells or red blood cell fragments are separated from other cell components in the patient sample by removing the paramagnetic beads from the specimen container by the magnet after the magnetic beads have selectively bound to the red blood cells or the red blood cell fragments.

16. The method of claim 13 wherein the magnetic beads are paramagnetic.