Method of preparing a modified surface
First Claim
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1. A method of preparing a modified surface, comprising:
- providing a surface to be modified;
contacting the surface to be modified with a surface modifying composition, wherein the surface modifying composition comprises;
a first surface modifying agent coupled to a reactive moiety, and a second surface modifying agent not coupled to the reactive moiety; and
the first surface modifying agent and second surface modifying agent are present in the surface modifying composition at a ratio that produces the modified surface, wherein the reactive moieties are present on the modified surface at a density of between about 1 reactive moiety per 50,000 nm2 and 1 reactive moiety per 100 nm2; and
coupling an enzyme to the reactive moiety on the surface.
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Abstract
Reactive surfaces, substrates and methods of producing and using such substrates and surfaces are provided. The substrates and surfaces provide low density reactive groups preferably on an otherwise non-reactive surface for use in different applications including single molecule analyses.
93 Citations
12 Claims
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1. A method of preparing a modified surface, comprising:
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providing a surface to be modified; contacting the surface to be modified with a surface modifying composition, wherein the surface modifying composition comprises; a first surface modifying agent coupled to a reactive moiety, and a second surface modifying agent not coupled to the reactive moiety; and the first surface modifying agent and second surface modifying agent are present in the surface modifying composition at a ratio that produces the modified surface, wherein the reactive moieties are present on the modified surface at a density of between about 1 reactive moiety per 50,000 nm2 and 1 reactive moiety per 100 nm2; and coupling an enzyme to the reactive moiety on the surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification