In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
First Claim
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1. A method of in vitro fertilization of oocytes, comprising the steps of:
- a) obtaining oocytes of a non-human mammal;
b) obtaining sperm cells of said non-human mammal separated into an X-chromosome bearing population or a Y-chromosome bearing population;
c) combining said oocytes of said non-human mammal in a fertilization medium supplemented with an amount of non-essential amino acids with said sperm cells of either said X-chromosome bearing population or said Y-chromosome bearing population;
d) establishing said plurality of oocytes with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population in incubation conditions within an incubation element; and
e) fertilizing at least one of said oocytes of said non-human mammal with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing populations, said amount of non-essential amino acids sufficient to achieve an increased rate of development of the resulting at least one embryo.
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Abstract
An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).
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Citations
12 Claims
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1. A method of in vitro fertilization of oocytes, comprising the steps of:
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a) obtaining oocytes of a non-human mammal; b) obtaining sperm cells of said non-human mammal separated into an X-chromosome bearing population or a Y-chromosome bearing population; c) combining said oocytes of said non-human mammal in a fertilization medium supplemented with an amount of non-essential amino acids with said sperm cells of either said X-chromosome bearing population or said Y-chromosome bearing population; d) establishing said plurality of oocytes with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population in incubation conditions within an incubation element; and e) fertilizing at least one of said oocytes of said non-human mammal with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing populations, said amount of non-essential amino acids sufficient to achieve an increased rate of development of the resulting at least one embryo. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification