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In-vitro fertilization systems with spermatozoa separated into X-chromosome and Y-chromosome bearing populations

  • US 8,137,967 B2
  • Filed: 08/21/2006
  • Issued: 03/20/2012
  • Est. Priority Date: 11/29/2000
  • Status: Expired due to Term
First Claim
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1. A method of in vitro fertilization of oocytes, comprising the steps of:

  • a) obtaining oocytes of a non-human mammal;

    b) obtaining sperm cells of said non-human mammal separated into an X-chromosome bearing population or a Y-chromosome bearing population;

    c) combining said oocytes of said non-human mammal in a fertilization medium supplemented with an amount of non-essential amino acids with said sperm cells of either said X-chromosome bearing population or said Y-chromosome bearing population;

    d) establishing said plurality of oocytes with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing population in incubation conditions within an incubation element; and

    e) fertilizing at least one of said oocytes of said non-human mammal with said sperm cells of said X-chromosome bearing population or said Y-chromosome bearing populations, said amount of non-essential amino acids sufficient to achieve an increased rate of development of the resulting at least one embryo.

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