Method of maintaining a protein concentration at a tangential flow filter

• US 8,137,983 B2
• Filed: 10/17/2011
• Issued: 03/20/2012
• Est. Priority Date: 04/12/2006
• Status: Active Grant

• Current Assignee: EMD Millipore Corporation (Merck & Co., Inc.)
• Original Assignee: EMD Millipore Corporation (Merck & Co., Inc.)
• Inventors: DiLeo, Anthony
• Primary Examiner(s): White, Dennis M
• Application Number:
  US13/274,386
• Publication Number:
  US 20120031839A1
• Time in Patent Office: 155 Days
• Field of Search: 436/3, 436/149, 436/164, 436/177, 422/119, 422/68.1, 422/82.05, 422/82.06, 422/82.07, 422/82.08, 422/82.09, 422/82.11, 422/83, 435/283.1, 435/287.1, 435/287.2, 435/287.7, 435/287.8, 435/287.9, 435/288.7, 435/295.3, 435/297.1, 435/297.3, 210/178, 210/198.1, 210/304, 210/512.1, 210/636, 210/639, 210/650, 210/652, 210/739, 210/741, 210/788, 210/905, 210/85, 210/90, 250/302, 250/343, 324/663, 324/698, 702/188, 702/50, 702/190, 73/616.3, 73/616.9, 731/147.7, 73/38, 73/407, 73/7, 738/614.2
• US Class Current: 436/177
• CPC Class Codes: A61M 2205/7509 for virus
• CPC Class Codes: B01D 2201/291 End caps
• CPC Class Codes: B01D 2311/08 Specific process operations in the concentrate stream
• CPC Class Codes: B01D 2311/14 Pressure control
• CPC Class Codes: B01D 2311/246 Concentration control
• CPC Class Codes: B01D 29/60 integrally combined with devices for controlling the filtration
• CPC Class Codes: B01D 35/143 Filter condition indicators
• CPC Class Codes: B01D 61/22 Controlling or regulating
• CPC Class Codes: B01D 65/104 Detection of leaks in membrane apparatus or modules
• CPC Class Codes: G01N 15/0618 of the filter type G01N15/0643 takes precedence
• CPC Class Codes: G01N 2015/084 Testing filters
• CPC Class Codes: G01N 2035/00811 consumable or exchangeable components other than sample carriers, e.g. detectors, flow cells
• CPC Class Codes: Y10S 210/905 Protein
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

The present invention describes a system and method for accurately measuring the concentration of a substance within a filter housing. A concentration sensor and a communications device are coupled so as to be able to measure and transmit the concentration of a particular substance within the filter housing while in use. This system can comprise a single component, integrating both the communication device and the concentration sensor. Alternatively, the system can comprise separate sensor and transmitter components, in communication with one another. In yet another embodiment, a storage element can be added to the system, thereby allowing the device to store a set of concentration values. The use of this device is beneficial to many applications. For example, the ability to read concentration values in situ allows integrity tests to be performed without additional equipment.

Claims

1. A method of maintaining a protein concentration at the membrane surface of a tangential flow filtering element within a filter housing, comprising:

attaching a concentration sensor to the membrane surface of said filtering element;

monitoring the concentration of said protein using said sensor;

comparing said concentration to a predetermined range; and

adjusting the transmembrane pressure in response to said comparison.

2. The method of claim 1, wherein said concentration sensor is in communication with a transmitter, further comprising the step of transmitting said monitored concentration to a receiver located outside of said housing said transmitter.

3. The method of claim 2, wherein said transmitter utilizes wireless communication.

4. The method of claim 3, wherein said wireless transmitter comprises an RFID tag.

5. The method of claim 1, wherein said adjustment is calculated using a PID loop.