Two-dimensional spectral imaging system
First Claim
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1. A system for sample analysis, comprising:
- an examination area including a two-dimensional array of examination sites;
a light detector for imaging;
an optical relay structure that spectrally disperses light received from the examination sites into a corresponding two-dimensional array of spectra directed concurrently onto the light detector in a collectively nonoverlapping relation wherein the optical relay structure includes one or more filters that truncate a portion of each spectra to substantially reduce crosstalk between the spectra of the two-dimensional array when directed concurrently onto the light detector; and
a thermal control system operatively coupled to the examination area for regulating a temperature thereof.
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Abstract
Systems, including methods, apparatus, and algorithms, for spectrally imaging a two-dimensional array of samples.
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Citations
12 Claims
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1. A system for sample analysis, comprising:
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an examination area including a two-dimensional array of examination sites; a light detector for imaging; an optical relay structure that spectrally disperses light received from the examination sites into a corresponding two-dimensional array of spectra directed concurrently onto the light detector in a collectively nonoverlapping relation wherein the optical relay structure includes one or more filters that truncate a portion of each spectra to substantially reduce crosstalk between the spectra of the two-dimensional array when directed concurrently onto the light detector; and a thermal control system operatively coupled to the examination area for regulating a temperature thereof. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11)
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2. A system for sample analysis, comprising:
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an examination area including a two-dimensional array of examination sites; a light detector for imaging; a controller operatively coupled to the light detector and configured to process image data collected by the light detector to correct for optical distortion in the image data; an optical relay structure that spectrally disperses light received from the examination sites into a corresponding two-dimensional array of spectra directed concurrently onto the light detector in a collectively nonoverlapping relation wherein the optical relay structure includes one or more filters that truncate a portion of each spectra to substantially reduce crosstalk between the spectra of the two-dimensional array when directed concurrently onto the light detector; and a thermal control system operatively coupled to the examination area for regulating a temperature thereof.
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12. A method of sample analysis, comprising:
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obtaining a two-dimensional array of samples for performing amplification of nucleic acids; cycling the samples thermally through a plurality of cycles; illuminating each sample of the array concurrently such that the samples emit light; directing dispersed light from each sample through at least one filter to a detection area such that a corresponding two-dimensional array of spectra falls concurrently onto the detection area and a portion of each spectra is truncated at the detector, wherein each spectra of the array of the spectra is non-overlapping with the remaining spectra of the array of the spectra; monitoring the two-dimensional array of spectra over time; and determining an aspect of one or more of the samples based on a measurement of the corresponding two-dimensional array of spectra.
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Specification