Electrochemical sensor

• US 8,142,641 B2
• Filed: 08/31/2006
• Issued: 03/27/2012
• Est. Priority Date: 09/01/2005
• Status: Active Grant

• Current Assignee: Palintest Limited (Halma PLC)
• Original Assignee: Palintest Limited (Halma PLC)
• Inventors: Birch, Stephen William, Sutherland, John
• Primary Examiner(s): Barton, Jeffrey T
• Assistant Examiner(s): Kessel, Maris R
• Application Number:
  US11/991,191
• Publication Number:
  US 20090321278A1
• Time in Patent Office: 2,035 Days
• Field of Search: 205/775, 2057785-7795, 204/435, 204/400
• US Class Current: 205/778.5
• CPC Class Codes: G01N 27/307 Disposable laminated or multilayered electrodes G01N27/3272 takes precedence
• CPC Class Codes: G01N 27/4045 for gases other than oxygen
• CPC Class Codes: G01N 33/0052 for gaseous halogens

Abstract

A electrochemical sensor comprising a mounting having screen printed array of electrodes located thereon, the array comprising a reference electrode, a counter electrode and a plurality of working electrodes, wherein the working electrodes are each overlaid with an insulating layer of insulating material, the insulating layer having an array of apertures, exposing a respective array of working regions of the working electrodes, and a method of making same by applying screen printing technic, and a method of using same for chlorine determination.

Claims

1. An electrochemical sensor comprising a mounting having a screen printed array of electrodes located thereon, the array comprising a reference electrode, a counter electrode, and a plurality of working electrodes,

wherein the working electrodes include a first working, electrode and a second working electrode that are each overlaid with an insulating layer of insulating material, the insulating layer of each of the first and second electrodes having an array of apertures exposing a respective array of working regions of the working electrodes,

wherein the first working electrode includes a first reagent layer that overlies the apertures within the insulating layer, the first reagent layer being impregnated with potassium iodide and a phthalate buffer, and

the second working electrode includes a second reagent layer that overlies the apertures within the insulating layer, the second reagent layer being impregnated with TMB and a buffer.

2. The sensor as claimed in claim 1, the sensor being operable for at least one of detection and assay of an analyte.

3. The sensor as claimed in claim 2, wherein the analyte is at least one form of chlorine.

4. The sensor as claimed in claim 3, wherein the sensor is operable to simultaneously determine the presence of at least one of free and total chlorine and free and combined chlorine.

5. The sensor as claimed in claim 1, wherein a conductive polymer is present on the working electrodes or a conductive polymer layer has been printed onto the sensor containing conductive ink as the working electrodes.

6. The sensor as claimed in claim 1, wherein the working electrodes include carbon.

7. The sensor as claimed in claim 6, wherein the working electrodes are screen printed.

8. The sensor as claimed in claim 1, wherein the reference electrode, two working electrodes and the counter electrode are arranged as four generally parallel tracks.

9. The sensor as claimed in claim 1, wherein the sensor is disposable by the inclusion therein of a fuseable ink.

10. The sensor as claimed in claim 1, further comprising a laminar sheet or strip of insulating polymeric material upon which successive layers have been applied by screen printing.

11. The sensor as claimed in claim 1, wherein the apertures in the array have a dimension of 50 to 400 IJM.

12. The sensor as claimed in claim 11, wherein the dimension is 100 to 200 μ

M.

13. The sensor as claimed in claim 1, wherein the apertures are circular.

14. The sensor as claimed in claim 1, wherein the array comprises 100 to 500 apertures.

15. The sensor as claimed in claim 14, wherein the array comprises 50 to 200 apertures.

16. The sensor as claimed in claim 9, wherein the array comprises 80 to 120 apertures.

17. The sensor as claimed in claim 16, wherein the array comprises 95 apertures.

18. The sensor as claimed in claim 1, wherein a plurality of tracks of highly conductive printable ink have been screen printed onto the sensor, each in contact with an electrical contact of the sensor.

19. The sensor as claimed in claim 18, wherein the ink includes either silver or silver chloride.

20. The sensor as claimed in claim 18, wherein the working electrodes deposited on the tracks include carbon or gold.

21. The sensor as claimed in claim 1, wherein the reagent layers each comprise a porous layer.

22. The sensor as claimed in claim 1, wherein the sensor is operable as an assay of total chlorine present in a test solution.

23. The sensor as claimed in claim 1, wherein the phthalate buffer is potassium acid phthalate.

24. The sensor as claimed in claim 1, wherein the buffer is a phosphate buffer.

25. The sensor as claimed in claim 24, wherein the reagent layers each further comprises comprise at least one of polyvinylpyrrolidone and hydroxyethylcellulose.

26. The sensor as claimed in claim 1, wherein the reagent layers are deposited by screen printing or microdosing.