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Small volume in vitro analyte sensor

  • US 8,142,642 B2
  • Filed: 07/30/2008
  • Issued: 03/27/2012
  • Est. Priority Date: 02/06/1997
  • Status: Expired due to Fees
First Claim
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1. A method for determining a concentration of an analyte in a body fluid of a patient, comprising the steps of:

  • piercing a portion of the skin of a patient to create a flow of a body fluid;

    transporting 500 nl or less of the body fluid into an analyte sensor comprising two pairs of electrodes and a redox mediator, each pair of electrodes comprising a working electrode and a reference or counter electrode, wherein the sensor is configured and arranged for determining the concentration of the analyte from 500 nL or less of body fluid;

    holding the sample within the analyte sensor in a non-flow through manner such that the sample is in electrolytic contact with the pairs of electrodes; and

    determining by coulomtery the concentration of the analyte in the body fluid from the 500 nl or less of body fluid transported into the analyte sensor.

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