×

Extraction with fractionation of oil and proteinaceous material from oleaginous material

  • US 8,142,659 B2
  • Filed: 04/06/2011
  • Issued: 03/27/2012
  • Est. Priority Date: 04/06/2010
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:

  • a. combining a wet algal biomass with an amphiphilic solvent set, the solvent set consisting of one or more amphiphilic solvents, to make a first extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the wet algal biomass;

    b. separating the first extraction mixture into a first liquid fraction comprising proteins and a first biomass fraction;

    c. combining the first biomass fraction with an amphiphilic solvent set to make a second extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the first biomass fraction;

    d. separating the second extraction mixture into a second liquid fraction comprising polar lipids and a second biomass fraction;

    e. combining the second biomass fraction with an amphiphilic solvent set to make a third extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the second biomass fraction;

    f. separating the third extraction mixture into a third liquid fraction comprising neutral lipids and a third biomass fraction, wherein the first liquid fraction has a higher protein concentration than the second and third liquid fractions, the second liquid fraction has a higher polar lipids concentration than the first and third liquid fractions, and the third liquid fraction has a higher neutral lipids concentration than the first and second liquid fractions.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×