Extraction with fractionation of oil and proteinaceous material from oleaginous material
First Claim
Patent Images
1. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:
- a. combining a wet algal biomass with an amphiphilic solvent set, the solvent set consisting of one or more amphiphilic solvents, to make a first extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the wet algal biomass;
b. separating the first extraction mixture into a first liquid fraction comprising proteins and a first biomass fraction;
c. combining the first biomass fraction with an amphiphilic solvent set to make a second extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the first biomass fraction;
d. separating the second extraction mixture into a second liquid fraction comprising polar lipids and a second biomass fraction;
e. combining the second biomass fraction with an amphiphilic solvent set to make a third extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the second biomass fraction;
f. separating the third extraction mixture into a third liquid fraction comprising neutral lipids and a third biomass fraction, wherein the first liquid fraction has a higher protein concentration than the second and third liquid fractions, the second liquid fraction has a higher polar lipids concentration than the first and third liquid fractions, and the third liquid fraction has a higher neutral lipids concentration than the first and second liquid fractions.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods for selective extraction and fractionation of algal lipids and algal products are disclosed. A method of selective removal of products from an algal biomass provides for single and multistep extraction processes which enable efficient separation of algal components. Among these components are neutral lipids synthesized by algae, which are extracted by the methods disclosed herein for the production of renewable fuels.
-
Citations
14 Claims
-
1. A method of selectively separating products from a wet algal biomass comprised of intact algal cells, the method comprising:
-
a. combining a wet algal biomass with an amphiphilic solvent set, the solvent set consisting of one or more amphiphilic solvents, to make a first extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the wet algal biomass; b. separating the first extraction mixture into a first liquid fraction comprising proteins and a first biomass fraction; c. combining the first biomass fraction with an amphiphilic solvent set to make a second extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the first biomass fraction; d. separating the second extraction mixture into a second liquid fraction comprising polar lipids and a second biomass fraction; e. combining the second biomass fraction with an amphiphilic solvent set to make a third extraction mixture, wherein the weight of the amphiphilic solvent set is about the same as the weight of the second biomass fraction; f. separating the third extraction mixture into a third liquid fraction comprising neutral lipids and a third biomass fraction, wherein the first liquid fraction has a higher protein concentration than the second and third liquid fractions, the second liquid fraction has a higher polar lipids concentration than the first and third liquid fractions, and the third liquid fraction has a higher neutral lipids concentration than the first and second liquid fractions. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
-
Specification