Mutant Citrobacter freundii phytase polypeptide
First Claim
1. An isolated polypeptide comprising an amino acid sequence corresponding to Citrobacter freundii phytase, wherein the polypeptide has phytase activity and wherein the polypeptide comprises the amino acid sequence as shown in SEQ ID NO:
- 3 or a sequence having at least 90% identity thereto and wherein said polypeptide comprises the mutation R288M according to the numbering in SEQ ID NO;
3.
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Accused Products
Abstract
The present invention relates to enzymes and processes. In particular, there is described an isolated polypeptide comprising the amino acid sequence corresponding to Citrobacter freundii phytase or a homologue, a modified form, a functional equivalent or an effective fragment thereof. There is also described a host cell transformed or transfected with a nucleic acid encoding a bacterial phytase enzyme or a modified form as well as the use of such a phytase or modified form in food or animal feed.
84 Citations
10 Claims
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1. An isolated polypeptide comprising an amino acid sequence corresponding to Citrobacter freundii phytase, wherein the polypeptide has phytase activity and wherein the polypeptide comprises the amino acid sequence as shown in SEQ ID NO:
- 3 or a sequence having at least 90% identity thereto and wherein said polypeptide comprises the mutation R288M according to the numbering in SEQ ID NO;
3. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10)
- 3 or a sequence having at least 90% identity thereto and wherein said polypeptide comprises the mutation R288M according to the numbering in SEQ ID NO;
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2. An isolated phytase isolated from Citrobacter freundii strain P3-42, deposited under accession number NCIMB 41247.
Specification
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- Resources
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Current AssigneeDuPont Nutrition Biosciences ApS (International Flavors & Fragrances Incorporated)
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Original AssigneeDanisco A/S (International Flavors & Fragrances Incorporated)
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InventorsLeuthner, Birgitta, Kettling, Ulrich, Koltermann, Andre, Miasnikov, Andrei, Kumar, Vijay, Kensch, Oliver, Pellangahr, Klaus
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Primary Examiner(s)Hutson, Richard
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Application NumberUS11/696,162Publication NumberTime in Patent Office1,820 DaysField of SearchNoneUS Class Current435/196CPC Class CodesC12N 9/16 acting on ester bonds (3.1)
