Compositions and methods for genetic modification of plants
First Claim
1. A soybean plant cell having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine in said soybean plant cell with their identical recombination site in the presence of an appropriate recombinase, wherein said first and said second non-identical recombination site comprises a FRT site or a mutant FRT site.
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Abstract
Methods for the targeted integration of nucleotide sequences into a plant are provided. Transfer cassettes comprising nucleotide sequences of interest flanked by non-identical recombination sites are used to transform a plant comprising a target site. The target site contains at least a set of non-identical recombination sites corresponding to those on the transfer cassette. Exchange of the nucleotide sequences flanked by the recombination sites is effected by a recombinase.
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Citations
14 Claims
- 1. A soybean plant cell having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine in said soybean plant cell with their identical recombination site in the presence of an appropriate recombinase, wherein said first and said second non-identical recombination site comprises a FRT site or a mutant FRT site.
- 8. A soybean plant having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine in said soybean plant with their identical recombination site in the presence of an appropriate recombinase, wherein said first and said second non-identical recombination site comprises a FRT site or a mutant FRT site.
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12. A transformed soybean seed having stably incorporated into its genome a transfer cassette comprising a nucleotide sequence of interest flanked by a first and a second non-identical recombination site, wherein said first and said second non-identical recombination site can recombine with their identical recombination site in the presence of an appropriate recombinase, wherein said first and said second non-identical recombination site comprises a FRT site or a mutant FRT site.
Specification