3D imaging of live cells with ultraviolet radiation
First Claim
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1. A system for 3D imaging of live cells in an optical tomography system comprising:
- a microfluidics cartridge including a tube positioned relative to a microscope objective, a conduit loop having a first port coupled to an entrance valve, a second port coupled to an exit valve, a semi-permeable membrane portion, a rotating portion and an imaging window;
where the rotating portion is mounted between a first fitting and a second fitting, where the first fitting couples the rotating portion to the entrance valve and the second fitting couples the rotating portion to the exit valve;
a rotation mechanism attached to the rotating portion;
a microscope objective located to view objects through the imaging window;
an axial translation mechanism coupled to the microscope objective;
a second conduit interfacing with the semi-permeable membrane, where the second conduit carries nutrients into the conduit loop and waste products out of the conduit loop;
at least one radiation source positioned to illuminate the imaging window including a biological object held therein, where the at least one radiation source generates radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm;
at least one sensor positioned to sense radiation transmitted through the biological object and the microscope objective;
an image processor coupled to receive data from the sensor; and
a reconstruction module coupled to the image processor, where the reconstruction module processes the data to form a 3D image of the biological object.
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Abstract
A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.
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Citations
38 Claims
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1. A system for 3D imaging of live cells in an optical tomography system comprising:
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a microfluidics cartridge including a tube positioned relative to a microscope objective, a conduit loop having a first port coupled to an entrance valve, a second port coupled to an exit valve, a semi-permeable membrane portion, a rotating portion and an imaging window; where the rotating portion is mounted between a first fitting and a second fitting, where the first fitting couples the rotating portion to the entrance valve and the second fitting couples the rotating portion to the exit valve; a rotation mechanism attached to the rotating portion; a microscope objective located to view objects through the imaging window; an axial translation mechanism coupled to the microscope objective; a second conduit interfacing with the semi-permeable membrane, where the second conduit carries nutrients into the conduit loop and waste products out of the conduit loop; at least one radiation source positioned to illuminate the imaging window including a biological object held therein, where the at least one radiation source generates radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm; at least one sensor positioned to sense radiation transmitted through the biological object and the microscope objective; an image processor coupled to receive data from the sensor; and a reconstruction module coupled to the image processor, where the reconstruction module processes the data to form a 3D image of the biological object. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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33. An optical tomography method including separate imaging stages along the same pathway comprising:
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transporting a plurality of biological objects along a pathway to a first stage; illuminating at least one object of the plurality of objects with visible light at the first stage to produce a diffraction pattern; sensing the diffraction pattern; analyzing the diffraction pattern to produce a diffraction analysis; illuminating the at least one object with visible light at a second stage; sensing visible light emanating from the at least one object to produce a first plurality of pseudoprojection images; illuminating the at least one object with DUV light at a first wavelength at a third stage; sensing the DUV light at a first wavelength emanating from the at least one object to produce a second plurality of pseudoprojection images; illuminating the at least one object with DUV light at a second wavelength at a fourth stage; sensing the DUV light at a second wavelength emanating from the at least one object to produce a third plurality of pseudoprojection images; sorting the at least one object responsively to the first, second and third pluralities of pseudoprojection images and the diffraction analysis. - View Dependent Claims (34, 35, 36, 37, 38)
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Specification