Method and apparatus for modifying pressure within a fluid dispenser

• US 8,147,773 B2
• Filed: 03/17/2011
• Issued: 04/03/2012
• Est. Priority Date: 08/11/1997
• Status: Expired due to Fees

• Current Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Original Assignee: Ventana Medical Systems Incorporated (Roche Holding AG)
• Inventors: Lemme, Charles D., Rizzo, Vincent R. Jr., Druyor-Sanchez, Bobbi, Ford, Anthony, Heilman, Bronwen, McDaniel, Darin, McGraw, Brian, Mead, Stephen, Richards, William, Showalter, Wayne
• Primary Examiner(s): Gordon, Brian R
• Application Number:
  US13/050,707
• Publication Number:
  US 20110168744A1
• Time in Patent Office: 383 Days
• Field of Search: 422/501, 422/537, 422/538, 422/542, 422/546, 422/521, 422/527, 738/633.2, 738/64, 738/640.1, 738/640.3, 738/641.1, 738/641.3, 738/641.5, 738/641.6
• US Class Current: 422/501
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2001/317 spraying liquids onto surfaces
• CPC Class Codes: G01N 2035/1034 Transferring microquantities of liquid
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1016 Control of the volume dispensed or introduced

Abstract

A fluid dispenser useful in automated biological reaction systems comprising a barrel having a reservoir chamber, the barrel having an upper portion and a lower portion, a cap located on top of the upper portion of the barrel and connected to the reservoir chamber, the cap having an external surface with at least one hole therethrough, a valve comprising a biasing member and a hole sealer, the valve being located in the hole in the cap at the point where a downward force is applied against the cap, a coupler having a dispense chamber, the coupler being coaxial with the barrel and wherein the barrel lower portion moves into the dispense chamber during dispensing of fluid, and a vent located between the cap and the reservoir chamber, the vent including a vent opening and a vent material composed of oliophobic material.

Claims

1. A fluid dispenser comprising:

a barrel having a reservoir chamber, the barrel having an upper portion and a lower portion;

a cap located on top of the upper portion of the barrel and connected to the reservoir chamber, the cap having an external surface with at least one hole therethrough;

a valve comprising a biasing member and a hole sealer, the valve being located in the hole in the cap at the point where a downward force is applied against the cap;

a coupler having a dispense chamber, the coupler being coaxial with the barrel and wherein the barrel lower portion moves into the dispense chamber during dispensing of fluid; and

a vent located between the cap and the reservoir chamber, the vent including a vent opening and a vent material composed of oliophobic material.

2. The fluid dispenser of claim 1 wherein the valve is oriented with the biasing member contacting the external surface of the cap, and the hole sealer located in and in sealing relation to the hole.

3. The fluid dispenser of claim 1 wherein the valve comprises a single-piece umbrella-shaped valve having a biasing portion, a stem and a seal located in the cap hole.

4. The fluid dispenser of claim 1 wherein the hole sealer is a bulge substantially spherical in shape.

5. The fluid dispenser of claim 1 wherein the external cap surface further comprises a raised surface that mates with the biasing portion of the valve.

6. The fluid dispenser of claim 5 wherein the raised surface further comprises breaks in the raised surface that function as air passageways.

7. A fluid dispenser comprising:

a barrel having a reservoir chamber, the barrel having an upper portion and a lower portion;

a cap located on top of the upper portion of the barrel and connected to the reservoir chamber, the cap having an external surface with at least one hole therethrough;

a valve comprising a single-piece umbrella-shaped valve having a biasing portion, a stem and a seal located in the cap hole, the valve being located at the point where a downward force is applied against the cap;

a coupler having a dispense chamber, the coupler being coaxial with the barrel and wherein the barrel lower portion moves into the dispense chamber during dispensing of fluid; and

a vent located between the cap and the reservoir chamber, the vent including a vent opening and a vent material composed of oliophobic material.

8. The fluid dispenser of claim 7 wherein the valve is inserted through the cap hole, oriented with the biasing member contacting the external surface of the cap, and the hole sealer located in and in sealing relation to the hole.

9. The fluid dispenser of claim 7 wherein the hole sealer is a bulge substantially spherical in shape.

10. The fluid dispenser of claim 7 wherein the external cap surface further comprises a raised surface that mates with the biasing portion of the valve.

11. The fluid dispenser of claim 10 wherein the raised surface further comprises breaks in the raised surface that function as air passageways.

12. A fluid dispenser comprising:

a barrel having a reservoir chamber, the barrel having upper and lower portions;

a cap located on top of the upper portion of the barrel and connected to the reservoir chamber, the cap having an external surface with at least one hole therethrough, said cap further comprises a raised surface on the cap external surface that mates with the biasing portion of the valve;

a valve comprising a biasing member and a hole sealer, the valve being located in the hole in the cap at the point where a downward force is applied against the cap;

a coupler having a dispense chamber, the coupler being coaxial with the barrel and wherein the barrel lower portion moves into the dispense chamber during dispensing of fluid; and

a vent located between the cap and the reservoir chamber, the vent including a vent opening and a vent material composed of oliophobic material.

13. The fluid dispenser of claim 12 wherein the valve is oriented with the biasing member contacting the external surface of the cap, and the hole sealer located in and in sealing relation to the hole.

14. The fluid dispenser of claim 12 wherein the valve comprises a single-piece umbrella-shaped valve having a biasing portion, a stem and a seal located in the cap hole.

15. The fluid dispenser of claim 12 wherein the hole sealer is a bulge substantially spherical in shape.

16. The fluid dispenser of claim 12 wherein the raised surface further comprises breaks in the raised surface that function as air passageways.