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Methods for detection and quantification of analytes in complex mixtures

  • US 8,148,512 B2
  • Filed: 06/18/2010
  • Issued: 04/03/2012
  • Est. Priority Date: 07/03/2001
  • Status: Expired due to Term
First Claim
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1. A probe comprising:

  • (a) a region that selectively binds to a target nucleic acid analyte,(b) a region comprising a plurality of label-attachment positions linked together, each label-attachment position being of a predetermined nucleotide sequence, wherein each label-attachment position is attached to a corresponding predetermined label monomer, wherein each label monomer is spatially-separable for visualization and wherein each label monomer comprises a fluorescent moiety; and

    (c) a biotin tag;

    wherein said plurality of label-attachment positions is at least four label-attachment positions and at least 4 corresponding label monomers.

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