Flowcell systems for single molecule detection
First Claim
1. A nucleotide, said nucleotide having a detectable label attached to the terminal phosphate, wherein said detectable label is linked to said terminal phosphate with a linker comprising a peptide of 2 to 15 amino acids in length.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
125 Citations
22 Claims
- 1. A nucleotide, said nucleotide having a detectable label attached to the terminal phosphate, wherein said detectable label is linked to said terminal phosphate with a linker comprising a peptide of 2 to 15 amino acids in length.
Specification