Emulsion compositions

• US 8,153,402 B2
• Filed: 10/06/2009
• Issued: 04/10/2012
• Est. Priority Date: 11/16/2001
• Status: Expired due to Term

• Current Assignee: UK Research and Innovation
• Original Assignee: Medical Research Council (Government of United Kingdom)
• Inventors: Holliger, Phillip, Ghadessy, Farid
• Primary Examiner(s): Vogel, Nancy
• Application Number:
  US12/587,432
• Publication Number:
  US 20100159592A1
• Time in Patent Office: 917 Days
• Field of Search: None
• US Class Current: 435/91.2
• CPC Class Codes: A61K 47/24 containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
• CPC Class Codes: A61K 47/26 Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
• CPC Class Codes: A61K 47/44 Oils, fats or waxes according to two or more groups of A61K47/02-A61K47/42; Natural or modified natural oils, fats or waxes, e.g. castor oil, polyethoxylated castor oil, montan wax, lignite, shellac, rosin, beeswax or lanolin synthetic glycerides, e.g. medium-chain triglycerides, A61K47/14
• CPC Class Codes: A61K 9/107 Emulsions ; Emulsion preconcentrates; Micelles composition of emulsions A61K47/00
• CPC Class Codes: A61K 9/1676 having a drug-free core with discrete complete coating layer containing drug adsorbates of liquid drug formulations on inert powders without simultaneous granulation step A61K9/141; with further drug-free outer coating A61K9/5078; drug conjugated to non-active particles A61K47/6921
• CPC Class Codes: C12N 15/1075 by coupling phenotype to genotype, not provided for in other groups of this subclass
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 2527/125 Specific component of sample, medium or buffer

Abstract

An emulsion is useful in allowing a wide variety of gene products to be expressed via eukaryotic in vitro expression. The emulsion comprises a silicone based surfactant, a hydrophobic phase and a hydrophilic phase; wherein the hydrophilic phase comprises a plurality of compartments containing a functional in vitro eukaryotic expression system.

Claims

1. An emulsion comprising a surfactant, a hydrophobic phase and a hydrophilic phase comprising a plurality of microcapsules containing a system for increasing the effective concentration of a nucleic acid molecule, wherein the surfactant is a chemically inert silicone-based surfactant and wherein the microcapsules have a sphere of diameter between 0.1 μ

m and 10 μ

m.

2. The emulsion of claim 1 wherein the emulsion comprises a hydrophilic phase to hydrophobic phase ratio of 1 to 2.5.

3. The emulsion of claim 1 wherein the nucleic acid molecule is genomic DNA or cDNA.

4. The emulsion of claim 1 wherein the nucleic acid molecule comprises a tag selected from the group consisting of biotin, digoxigenin, and 2,4-dinitrophenyl,

5. The emulsion of claim 1, wherein the chemically inert silicone-based surfactant is a silicone copolymer.

6. The emulsion of claim 1, wherein the silicone copolymer is polysiloxane-polycetyl-polyethylene glycol copolymer.

7. The emulsion of claim 1, wherein the chemically inert silicone-based surfactant is Abil EM90.

8. The emulsion of claim 1, wherein the emulsion is thermostable.

9. The emulsion of claim 1 wherein the chemically inert silicone-based surfactant is provided at a v/v concentration range of 0.5-20% of surfactant in oil.

10. The emulsion of claim 1 wherein the plurality of microcapsules when formed each contains on average one or less than one nucleic acid molecule.

11. The emulsion of claim 1 wherein the plurality of microcapsules when formed each contains on average between 5 and 1000 nucleic acid molecules.