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Oligonucleotide probes and primers comprising universal bases for diagnostic purposes

  • US 8,153,772 B2
  • Filed: 02/25/2003
  • Issued: 04/10/2012
  • Est. Priority Date: 10/02/1997
  • Status: Expired due to Term
First Claim
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1. An oligonucleotide probe for detecting a first target nucleic acid sequence comprising the formula:


  • XRYwherein X comprises between about 5 and 20 modified nucleic acid bases complementary to a first region of said first target nucleic acid sequence;

    wherein R comprises between about 5 and 10 universal or generic bases juxtaposed to one another and said universal or generic bases align with bases of a second region of said first target nucleic acid sequence;

    wherein Y comprises between about 5 and about 20 nucleic acid bases complementary to a third region of said first target nucleic acid sequence;

    wherein X, R, and Y are covalently joined;

    wherein greater than 20% of the total number of bases in said oligonucleotide probe are said universal or generic bases juxtaposed to one another;

    wherein said oligonucleotide probe further comprises a detectable label; and

    wherein said oligonucleotide probe is capable of hybridizing to and detecting said first target nucleic acid sequence but not able to detect a second target nucleic acid sequence that differs from said first target nucleic acid sequence by a single nucleotide base mismatch in the first or third region of the target nucleic acid sequence.

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