Device and method for particle complex handling

• US 8,158,008 B2
• Filed: 08/04/2011
• Issued: 04/17/2012
• Est. Priority Date: 12/29/2006
• Status: Active Grant

• Current Assignee: Intel Corporation
• Original Assignee: Intel Corporation
• Inventors: Su, Xing, Liu, David J., Swartz, Kenneth B., Wu, Kai, Yamakawa, Mineo
• Primary Examiner(s): Reifsnyder, David A
• Application Number:
  US13/197,918
• Publication Number:
  US 20120035084A1
• Time in Patent Office: 257 Days
• Field of Search: 210/222, 210/223, 210/695, 436/164, 436/526, 436/805, 436/806, 435/287.2, 435/287.3, 435/288.4, 435/288.5, 422/400, 422/82.05, 73/530.1
• US Class Current: 210/695
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0424 Dielectrophoretic forces
• CPC Class Codes: B01L 2400/043 magnetic forces
• CPC Class Codes: B01L 2400/0638 membrane valves, flap valves
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 3/502761 specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules investigating characteristics of particles G01N15/00
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: G01N 1/4044 by chemical techniques; Digestion; Chemical decomposition
• CPC Class Codes: G01N 2015/0038 Investigating nanoparticles
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: H01F 2007/068 using printed circuit coils
• CPC Class Codes: H01F 7/20 without armatures cores H01F3/00; coils H01F5/00 ; shaping metal by applying magnetic forces B21D26/14; electromagnets specially adapted for NMR applications G01R33/381
• CPC Class Codes: H01F 7/206 Electromagnets for lifting, handling or transporting of magnetic pieces or material electromagnets for guidance of vehicles, workpieces B65G21/2009; for magnetic suspension or levitation H02N15/00
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10T 29/49117 Conductor or circuit manufacturing
• CPC Class Codes: Y10T 29/4913 : Assembling to base an electrical component, e.g., capacitor, etc.
• CPC Class Codes: Y10T 29/49155 : Manufacturing circuit on or in base
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

An embodiment of the invention relates to a device for detecting an analyte in a sample. The device comprises a fluidic network and an integrated circuitry component. The fluidic network comprises a sample zone, a cleaning zone and a detection zone. The fluidic network contains a magnetic particle and/or a signal particle. A sample containing an analyte is introduced, and the analyte interacts with the magnetic particle and/or the signal particle through affinity agents. A microcoil array or a mechanically movable permanent magnet is functionally coupled to the fluidic network, which are activatable to generate a magnetic field within a portion of the fluidic network, and move the magnetic particle from the sample zone to the detection zone. A detection element is present which detects optical or electrical signals from the signal particle, thus indicating the presence of the analyte.

Claims

1. A device comprising:

a substrate containing a plurality of fluidic zones, each fluidic zone being connected to the adjacent fluidic zone by a diffusion barrier, wherein each fluidic zone comprises a fluid, wherein one or more fluidic zones comprises a magnetic particle, and wherein the device is adapted to permit transport of the magnetic particle from one fluidic zone to an adjacent fluidic zone substantially without moving the fluid between the fluidic zones.

2. The device of claim 1, wherein the diffusion barrier is a fluidic channel and/or a thermally-sensitive barrier.

3. The device of claim 1, wherein the diffusion barrier is a hydrophilic-hydrophobic interface, created by completely surrounding the hydrophilic reagents by hydrophobic liquid.

4. The device of claim 1, wherein the diffusion barrier is created by a MEMS membrane valve.

5. The device of claim 1, wherein the diffusion barrier is created by dielectric phoresis.

6. The device of claim 1, wherein the magnetic particle is a magnetic affinity complex, a competitive binding complex and/or a coded magnetic affinity complex.

7. The device of claim 1, wherein the plurality of fluidic zones comprises a sample zone, a cleaning zone, and a detection zone.

8. The device of claim 7, wherein the sample zone comprises a space for holding a sample, and is selected from a reservoir, a channel, an opening, a surface, or a combination thereof.

9. The device of claim 7, further comprising an additional fluidic zone adapted for the storage of one or more reagents.

10. The device of claim 7, wherein the detection zone comprises a reaction substrate that is capable of interacting with a catalytic element to form a reaction product.

11. The device of claim 1, wherein multiple sets of fluidic zones are present in parallel in the device.

12. The device of claim 1, wherein one or more fluidic zones comprises a signal affinity complex.

13. The device of claim 1, further comprising an integrated circuitry component.