Transgenic non-human animals for producing chimeric antibodies
First Claim
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1. An isolated B lymphocyte hybridoma clone wherein the B lymphocyte hybridoma clone expresses an antibody comprising a heavy chain consisting of a human immunoglobulin heavy chain variable region and a mouse immunoglobulin heavy chain gamma constant region, wherein the isolated B lymphocyte hybridoma is derived from a fusion of:
- (a) a B lymphocyte from a transgenic mouse, wherein the genome of said transgenic mouse comprises inactivated murine heavy chain variable regions and an unrearranged human heavy chain immunoglobulin variable region operably linked to a mu constant region gene segment, wherein the unrearranged heavy chain variable region comprises multiple human VH gene segments, multiple human D gene segments, and multiple human JH gene segments, and wherein the mu constant region is selected from the group consisting of;
(i) a human S-mu sequence and a human or mouse mu coding sequence; and
(ii) a mouse S-mu sequence and a human or mouse mu coding sequence; and
(b) a myeloma cell.
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Abstract
The invention relates to transgenic non-human animals capable of producing heterologous antibodies and methods for producing human sequence antibodies which bind to human antigens with substantial affinity.
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Citations
2 Claims
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1. An isolated B lymphocyte hybridoma clone wherein the B lymphocyte hybridoma clone expresses an antibody comprising a heavy chain consisting of a human immunoglobulin heavy chain variable region and a mouse immunoglobulin heavy chain gamma constant region, wherein the isolated B lymphocyte hybridoma is derived from a fusion of:
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(a) a B lymphocyte from a transgenic mouse, wherein the genome of said transgenic mouse comprises inactivated murine heavy chain variable regions and an unrearranged human heavy chain immunoglobulin variable region operably linked to a mu constant region gene segment, wherein the unrearranged heavy chain variable region comprises multiple human VH gene segments, multiple human D gene segments, and multiple human JH gene segments, and wherein the mu constant region is selected from the group consisting of; (i) a human S-mu sequence and a human or mouse mu coding sequence; and (ii) a mouse S-mu sequence and a human or mouse mu coding sequence; and (b) a myeloma cell. - View Dependent Claims (2)
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Specification