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Transgenic non-human animals for producing chimeric antibodies

  • US 8,158,419 B2
  • Filed: 09/26/2008
  • Issued: 04/17/2012
  • Est. Priority Date: 03/09/1994
  • Status: Expired due to Fees
First Claim
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1. An isolated B lymphocyte hybridoma clone wherein the B lymphocyte hybridoma clone expresses an antibody comprising a heavy chain consisting of a human immunoglobulin heavy chain variable region and a mouse immunoglobulin heavy chain gamma constant region, wherein the isolated B lymphocyte hybridoma is derived from a fusion of:

  • (a) a B lymphocyte from a transgenic mouse, wherein the genome of said transgenic mouse comprises inactivated murine heavy chain variable regions and an unrearranged human heavy chain immunoglobulin variable region operably linked to a mu constant region gene segment, wherein the unrearranged heavy chain variable region comprises multiple human VH gene segments, multiple human D gene segments, and multiple human JH gene segments, and wherein the mu constant region is selected from the group consisting of;

    (i) a human S-mu sequence and a human or mouse mu coding sequence; and

    (ii) a mouse S-mu sequence and a human or mouse mu coding sequence; and

    (b) a myeloma cell.

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