Cell culture scaffold containing gel having interpenetrating polymer network structure

• US 8,158,425 B2
• Filed: 08/29/2008
• Issued: 04/17/2012
• Est. Priority Date: 06/30/2004
• Status: Expired due to Fees

• Current Assignee: Hokkaido University
• Original Assignee: Hokkaido University
• Inventors: Gong, Jian Ping, Osada, Yoshihito, Chen, Yongmei
• Primary Examiner(s): Naff, David
• Application Number:
  US12/230,457
• Publication Number:
  US 20090068739A1
• Time in Patent Office: 1,327 Days
• Field of Search: None
• US Class Current: 435/397
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing a synthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).

Claims

1. A cell culture scaffold containing a gel, the gel comprising an interpenetrating polymer network structure comprising a network structure comprised of a polymer and a crosslinked synthetic polymer,

wherein the network structure has a crosslinked structure;

the crosslinked synthetic polymer penetrates the network structure and is produced by polymerizing or copolymerizing a monomer composition which contains a p-styrenesulfonic acid alkali metal salt or a 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and a crosslinking agent and in which the network structure is immersed; and

the polymer constituting the network structure has no sulfonic group.

3. The cell culture scaffold according to claim 1 or 2, wherein the polymer constituting the network structure is a cellulose that is produced by bacteria.

4. A method for culturing cells comprising culturing cells on the scaffold according to claim 1 or 2.

2. A cell culture scaffold containing a gel, the gel comprising a semi-interpenetrating polymer network structure comprising a network structure comprised of a polymer and a linear synthetic polymer,

wherein the network structure has a crosslinked structure;

the linear synthetic polymer penetrates the network structure and is produced by polymerizing or copolymerizing a monomer composition which contains a p-styrenesulfonic acid alkali metal salt or a 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and in which the network structure is immersed; and

the polymer constituting the network structure has no sulfonic group.