Multiple operating mode sample analyzer, analyzing method, and computer program product

• US 8,158,439 B2
• Filed: 07/16/2008
• Issued: 04/17/2012
• Est. Priority Date: 07/31/2007
• Status: Active Grant

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Shibata, Masaharu
• Primary Examiner(s): Soderquist, Arlen
• Application Number:
  US12/218,607
• Publication Number:
  US 20090035873A1
• Time in Patent Office: 1,371 Days
• Field of Search: 422/67, 422/73, 422/514, 422 63- 65, 436/43, 436/63, 436179-180, 436/50, 436/55, 436/66
• US Class Current: 436/179
• CPC Class Codes: G01N 1/38 Diluting, dispersing or mixing samples
• CPC Class Codes: G01N 15/10 Investigating individual particles
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1477 Multiparameters
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 35/00584 Control arrangements for automatic analysers
• CPC Class Codes: G01N 35/1095 for supplying the samples to flow-through analysers for a specific analyser see relevant groups, e.g. under G01N15/00, G01N21/00, G01N27/00, G01N30/00, H01J49/00
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/12 Condition responsive control
• CPC Class Codes: Y10T 436/25625 Dilution
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

The present invention is to present a sample analyzer which is automated even when measuring a small amount sample and is capable of improving a measurement precision. The sample analyzer S comprises a sample preparation section for preparing a measurement sample; a detector D3 for detecting an analyte contained in the measurement sample; and a controller 100 being configured to 1) control the sample preparation section so as to prepare a first measurement sample of a first dilution ratio, when measuring an ordinary amount sample; and 2) control the sample preparation section so as to prepare a second measurement sample of a second dilution ratio higher than the first dilution ratio, when measuring a small amount sample.

Claims

1. A blood analyzer comprising:

a sample preparation section for preparing a measurement sample from an undiluted blood in a sample container and a reagent;

a detector comprising a flow cell for passing the measurement sample prepared by the sample preparation section and detecting blood components contained in the measurement sample passing through the flow cell;

a process control system having a command mode input configured to specify a first operating mode or a second operating mode; and

a controller in communication with the sample preparation section and configured to carry out the first operating mode or the second operating mode, where

1) in the first operating mode, the controller controls the sample preparation section so as to obtain a first amount of the undiluted blood from the sample container, and prepare a first measurement sample of a first dilution ratio, and the detector detects blood components contained in a first amount of the first measurement sample; and

2) in the second operating mode, the controller controls the sample preparation section so as to obtain a second amount of the undiluted blood from a sample container, the second amount of the undiluted blood being less than the first amount of the undiluted sample, and prepare a second measurement sample of a second dilution ratio higher than the first dilution ratio, and the detector detects blood components contained in a second amount of the second measurement sample larger than the first amount of the first measurement sample.

2. The blood analyzer of claim 1, further comprising

a sample supplier for supplying the measurement sample prepared by the sample preparation section to the flow cell, wherein

in the first operating mode, the controller controls the sample supplier so as to supply the first measurement sample to the flow cell for a first period;

and

2) in the second operating mode, the controller controls the sample supplier so as to supply the second measurement sample to the flow cell for a second period longer than the first period.

3. The blood analyzer of claim 2, wherein the controller is configured to control the sample supplier so as to supply the measurement samples prepared by the sample preparation section to the flow cell at a predetermined speed, regardless of whether in the first operating mode or the second operating mode.

4. The blood analyzer of claim 1, further comprising a sample supplier for supplying the measurement sample prepared by the sample preparation section to the flow cell, whereinin the first operating mode, the controller controls the sample supplier so as to supply the first amount of the first measurement sample to the flow cell;

and

2) in the second operating mode, the controller controls the sample supplier so as to supply the second amount of the second measurement sample to the flow cell.

5. The blood analyzer of claim 1, wherein the detector comprises:

a light emitting section for irradiating light on the measurement sample passing through the flow cell; and

a light receiving section for receiving light from the measurement sample irradiated by the light emitting section.

6. The blood analyzer of claim 1, wherein the controller is configured to control the sample preparation section so as to prepare the measurement sample using substantially a similar amount of the reagent, regardless of whether in the first operating mode or in the second operating mode.

7. A method of analyzing blood in a sample analyzing, the method comprising:

providing a sample preparation section for preparing a measurement sample from an undiluted blood in a sample container and a reagent;

providing a detector comprising a flow cell for passing the measurement sample prepared by the sample preparation section and detecting blood components contained in the measurement sample passing through the flow cell;

providing a process control system having a command mode input configured to specify a first operating mode or a second operating mode; and

providing a controller in communication with the sample preparation section and operating the analyzer to carry out the first operating mode or the second operating mode, where

1) in the first operating mode, the controller controls the sample preparation section so as to obtain a first amount of the undiluted blood from the sample container, and prepare a first measurement sample of a first dilution ratio, and the detector detects blood components contained in a first amount of the first measurement sample; and

2) in the second operating mode, the controller controls the sample preparation section so as to obtain a second amount of the undiluted blood from a sample container, the second amount of the undiluted blood being less than the first amount of the undiluted sample, and prepare a second measurement sample of a second dilution ratio higher than the first dilution ratio, and the detector detects blood components contained in a second amount of the second measurement sample larger than the first amount of the first measurement sample.

8. The method of claim 7, further comprising providing a sample supplier for supplying the measurement sample prepared by the sample preparation section to the flow cell, whereinin the first operating mode, the controller controls the sample supplier so as to supply the first measurement sample to the flow cell for a first period;

and

2) in the second operating mode, the controller controls the sample supplier so as to supply the second measurement sample to the flow cell for a second period longer than the first period.

9. The method of claim 8, wherein the controller controls the sample supplier so as to supply the measurement sample prepared by the sample preparation section to the flow cell at a predetermined speed, regardless of whether the controller is carrying out the first operating mode or the second operating mode.

10. The method of claim 7 further comprising providing a sample supplier for supplying the measurement sample prepared by the sample preparation section to the flow cell, whereinin the first operating mode, the controller controls the sample supplier so as to supply the first amount of the first measurement sample to the flow cell;

and

2) in the second operating mode, the controller controls the sample supplier so as to supply the second amount of the second measurement sample to the flow cell.

11. The blood analyzer of claim 7, wherein providing a detector comprises:

providing a light emitting section for irradiating light on the measurement sample passing through the flow cell; and

providing a light receiving section for receiving light from the measurement sample irradiated by the light emitting section.

12. The blood analyzer of claim 7, wherein the controller controls the sample preparation section so as to prepare the measurement sample using substantially a similar amount of the reagent, regardless of whether the controller is operating the analyzer in the first operating mode or in the second operating mode.

13. A computer program product for enabling a computer to execute a blood analyzing method in a blood analyzer which is capable of performing analysis of blood, comprising:

a computer readable medium including software instructions for enabling the computer to perform predetermined operations in a first operating mode or a second operating mode, the operations comprising;

in the first operating mode, obtaining a first amount of an undiluted blood from a sample container, and preparing a first measurement sample of a first dilution ratio from the obtained undiluted blood and detecting blood components contained in a first amount of the first measurement sample;

in the second operating mode, obtaining a second amount of the undiluted blood from a sample container, the second amount of undiluted blood being less than the first amount, and preparing a second measurement sample of a second dilution ratio higher than the first dilution ratio and detecting blood components contained in a second amount of the second measurement sample larger than the first amount of the first measurement sample.

14. The computer program product of claim 13, wherein

in the first operating mode, detecting blood components comprises detecting blood cells contained in the first measurement sample for a first period, and

in the second operating mode, detecting blood components comprises detecting blood cells contained in the second measurement sample for a second period longer than the first period.

15. The computer program product of claim 14 further comprising software instructions for supplying the measurement sample at a predetermined speed to a detector for detecting the analyte contained in the measurement sample, regardless of whether the measurement sample is from the first operating mode or the second operating mode.

16. The computer program product of claim 13, wherein detecting blood components comprises preparing measurement samples using a substantially similar amount of the reagent regardless of whether detecting blood components in the first operating mode or the second operating mode.