Confocal imaging methods and apparatus

• US 8,158,926 B2
• Filed: 05/02/2011
• Issued: 04/17/2012
• Est. Priority Date: 11/23/2005
• Status: Active Grant

• Current Assignee: Illumina Incorporated
• Original Assignee: Illumina Incorporated
• Inventors: Feng, Wenyi, Kotseroglou, Theofilos, Wang, Mark, Triener, Alexander, Che, Diping, Kain, Robert
• Primary Examiner(s): LEE, JOHN R
• Application Number:
  US13/099,062
• Publication Number:
  US 20110204212A1
• Time in Patent Office: 351 Days
• Field of Search: 250/208.1, 250/208.2, 250/458.1, 250/459.1, 250/586, 250/234, 250/235, 250/236
• US Class Current: 250/234
• CPC Class Codes: G01N 2021/6421 Measuring at two or more wavelengths
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G02B 21/0032 Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06
• CPC Class Codes: G02B 21/004 fixed arrays, e.g. switchable aperture arrays

Abstract

The invention provides imaging apparatus and methods useful for obtaining a high resolution image of a sample at rapid scan rates. A rectangular detector array having a horizontal dimension that is longer than the vertical dimension can be used along with imaging optics positioned to direct a rectangular image of a portion of a sample to the rectangular detector array. A scanning device can be configured to scan the sample in a scan-axis dimension, wherein the vertical dimension for the rectangular detector array and the shorter of the two rectangular dimensions for the image are in the scan-axis dimension, and wherein the vertical dimension for the rectangular detector array is short enough to achieve confocality in a single axis.

Claims

1. A detection apparatus comprising:

(a) a line generator positioned to send a radiation line to a sample region, wherein the shorter of two dimensions for the radiation line is between 0.05 micrometer and 10 micrometers;

(b) a rectangular detector array comprising a plurality of pixel elements in a horizontal axis and a plurality of pixel elements in a vertical axis, wherein the horizontal axis is longer than the vertical axis; and

(c) optics positioned to direct radiation from the sample region to the rectangular detector array,

wherein the rectangular detector array is configured to collect electrical responses from pixel elements in the vertical axis in a common bin, wherein the Rayleigh resolution of the detection apparatus is in the range of 0.1 micrometer to 10 micrometers.

2. The apparatus of claim 1, further comprising a radiation source positioned to send excitation radiation to the line generator.

3. The apparatus of claim 2, further comprising a second radiation source positioned to send a second radiation line to the sample region, thereby simultaneously forming spaced multi-lines with multi-colors on the sample region.

4. The apparatus of claim 3, further comprising a second rectangular detector array comprising a plurality of pixel elements in a horizontal axis and a plurality of pixel elements in a vertical axis, wherein the horizontal axis is longer than the vertical axis.

5. The apparatus of claim 1, further comprising a scanning device configured to scan the radiation line across the sample region in a scan-axis dimension, wherein the vertical axis for the rectangular detector array is in the scan-axis dimension.

6. The apparatus of claim 5, wherein the scanning device comprises a translation stage configured to move a sample in the scan-axis dimension.

7. The apparatus of claim 5, wherein the optics are positioned to direct the radiation to the rectangular detector array as a rectangular image of the sample region, and wherein the shorter of the two rectangular dimensions for the image correlates with the vertical axis of the rectangular array.

8. The apparatus of claim 7, wherein the shorter of the two rectangular dimensions for the image is in the scan axis dimension.

9. The apparatus of claim 1, wherein the aspect ratio of the rectangular detector array is greater than 20.

10. The apparatus of claim 1, wherein the sample region comprises a microarray comprising fluorescent molecules.

11. The apparatus of claim 1 wherein the rectangular detector array is further configured to collect electrical responses from pixel elements in the horizontal axis in a common bin.

12. The apparatus of claim 1, wherein the ratio of the shorter of the two dimensions for the radiation line to the Rayleigh resolution of the detection apparatus is in the range of 0.1 to 10.

13. The apparatus of claim 1, wherein the radiation line has a cross sectional shape that is rectangular.

14. A method of detecting a sample in a detection apparatus, comprising

(a) directing a radiation line to a sample, thereby exciting fluorophores to produce emission radiation, wherein the shorter of two dimensions for the radiation line is between 0.05 micrometer and 10 micrometers;

(b) directing the emission radiation from the sample to a rectangular detector array, wherein the rectangular detector array comprises a plurality of pixel elements in a horizontal axis and a plurality of pixel elements in a vertical axis, wherein the horizontal axis is longer than the vertical axis;

(c) collecting electrical responses from a plurality of pixel elements of the vertical axis in a common bin; and

(d) reading the collected electrical responses from the common bin as a single value, wherein the Rayleigh resolution of the detection apparatus is in the range of 0.1 micrometer to 10 micrometers.

15. The method of claim 14, further comprising directing a second radiation line to the sample, wherein the radiation line and the second radiation line excite different fluorophores to produce different emission radiation colors.

16. The method of claim 15, wherein the radiation line and the second radiation line form a spaced multi-line to simultaneously excite the different fluorophores and simultaneously produce a multi-line image comprising the different emission radiation colors.

17. The method of claim 16, wherein step (b) comprises directing the multi line image from the sample to multiple rectangular detector arrays, wherein each rectangular detector array comprises a plurality of pixel elements in a horizontal axis and a plurality of pixel elements in a vertical axis, wherein the horizontal axis is longer than the vertical axis.

18. The method of claim 17, further comprising scanning the radiation line across the sample in a scan-axis dimension, wherein the vertical axis for the rectangular detector array is in the scan-axis dimension.

19. The method of claim 18, wherein the scanning comprises moving the sample in the scan-axis dimension.

20. The method of claim 18, wherein the scanning comprises moving the radiation line in the scan-axis dimension.

21. The method of claim 18, wherein the emission radiation is directed to the rectangular detector array as a rectangular image of the sample, and wherein the shorter of the two rectangular dimensions for the image correlates with the vertical axis of the rectangular array.

22. The method of claim 21, wherein the shorter of the two rectangular dimensions for the image is in the scan axis dimension.

23. The method of claim 14, wherein the sample comprises a microarray having sites that are separated by less than 10 micrometers.

24. The method of claim 14, wherein the ratio of the shorter of the two dimensions for the radiation line to the Rayleigh resolution of the detection apparatus is in the range of 0.1 to 10.