Analyte monitoring: stabilizer for subcutaneous glucose sensor with incorporated antiglycolytic agent

• US 8,160,670 B2
• Filed: 07/03/2008
• Issued: 04/17/2012
• Est. Priority Date: 12/28/2005
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Ouyang, Tianmei, Liu, Zenghe, Cho, Hyun, Feldman, Benjamin J.
• Primary Examiner(s): MALLARI, PATRICIA C
• Application Number:
  US12/167,820
• Publication Number:
  US 20090018425A1
• Time in Patent Office: 1,384 Days
• Field of Search: 600/347, 600/365
• US Class Current: 600/347
• CPC Class Codes: A61B 5/0031 Implanted circuitry
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/1477 non-invasive
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors

Abstract

An analyte sensor including an antiglycolytic agent or a precursor thereof and a chelating agent that stabilizes the antiglycolytic agent positioned proximate to the working electrode of the sensor. Also provided are systems and methods of using the electrochemical analyte sensors in analyte monitoring.

Claims

1. An electrochemical analyte sensor, comprising:

a working electrode comprising a sensing layer disposed thereon;

a counter electrode; and

an antiglycolytic agent or precursor thereof chemically bonded to a chelating agent, wherein the antiglycolytic agent or precursor thereof and the chelating agent are disposed proximate to the working electrode.

2. The sensor of claim 1, wherein the antiglycolytic agent is fluoride, glyceraldehyde, mannose, glucosamine, mannoheptulose, sorbose-6-phophate, or trehalose-6-phosphate.

3. The sensor of claim 1, wherein the antiglycolytic agent is a glyceraldehyde or a precursor thereof.

4. The sensor of claim 1, wherein the chelating agent is boric acid.

5. The sensor of claim 1, wherein the chelating agent is a transition metal.

6. The sensor of claim 3, wherein the glyceraldehyde or precursor thereof is present in an amount that ranges from about 1 microgram to about 2 milligrams.

7. The sensor of claim 4, wherein the boric acid is present in an amount that ranges from about 1 microgram to about 2 milligrams.

8. The sensor of claim 1, wherein the sensor further comprises a membrane disposed over the sensing layer.

9. The sensor of claim 8, wherein the membrane comprises the antiglycolytic agent or precursor thereof and the chelating agent.

10. The sensor of claim 9, wherein the antiglycolytic agent is a glyceraldehyde or precursor thereof.

11. The sensor of claim 9, wherein the chelating agent is boric acid.

12. The sensor of claim 9, wherein the chelating agent is a transition metal.

13. The sensor of claim 9, wherein the antiglycolytic agent or precursor thereof and the chelating agent are disposed on a surface of the membrane.

14. The sensor of claim 9, wherein the antiglycolytic agent or precursor thereof and the chelating agent are incorporated into the membrane.

15. The sensor of claim 1, wherein the antiglycolytic agent or precursor thereof and the chelating agent are comprised in a time-release formulation.

16. The sensor of claim 1, wherein the antiglycolytic agent precursor further comprises an enzyme.

17. The sensor of claim 16, wherein the enzyme is trehalose-6-phosphate synthase.

18. The sensor of claim 1, wherein the sensing layer of the working electrode comprises a glucose-responsive enzyme.

19. The sensor of claim 1, wherein the sensing layer comprises a redox mediator.

20. The sensor of claim 19, wherein the redox mediator comprises a complex selected from the group consisting of a ruthenium-containing complex and an osmium-containing complex.