Eukaryotic Microorganisms for producing lipids and antioxidants

• US 8,163,515 B2
• Filed: 06/07/2006
• Issued: 04/24/2012
• Est. Priority Date: 06/07/2005
• Status: Active Grant

• Current Assignee: DSM Nutritional Products AG (DSM-Firmenich AG)
• Original Assignee: Ocean Nutrition Canada Limited (DSM-Firmenich AG)
• Inventors: Burja, Adam M., Radianingtyas, Helia, Barrow, Colin James, Windust, Anthony James
• Primary Examiner(s): Liu, Sue
• Assistant Examiner(s): Shen, Bin
• Application Number:
  US11/916,781
• Publication Number:
  US 20090117194A1
• Time in Patent Office: 2,148 Days
• Field of Search: 435/41
• US Class Current: 435/41
• CPC Class Codes: A21D 8/04 treating dough with microorganisms or enzymes
• CPC Class Codes: A23K 10/18 of live microorganisms
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23L 29/065 Microorganisms addition of bacteria for nutritional purposes A23L33/135
• CPC Class Codes: A23L 33/115 Fatty acids or derivatives thereof; Fats or oils
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23L 33/135 Bacteria or derivatives thereof, e.g. probiotics
• CPC Class Codes: A23P 10/30 Encapsulation of particles, e.g. foodstuff additives
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A23V 2200/02 Antioxidant
• CPC Class Codes: A23V 2200/3262 having an effect on blood cholesterol
• CPC Class Codes: A23V 2200/328 having effect on glycaemic control and diabetes
• CPC Class Codes: A23V 2200/332 Promoters of weight control and weight loss
• CPC Class Codes: A61K 31/20 having a carboxyl group bound to a chain of seven or more carbon atoms, e.g. stearic, palmitic, arachidic acids
• CPC Class Codes: A61K 9/4875 Compounds of unknown constitution, e.g. material from plants or animals oils, fats, waxes, shellac A61K9/4858
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 3/02 Nutrients, e.g. vitamins, minerals
• CPC Class Codes: A61P 3/04 Anorexiants; Antiobesity agents
• CPC Class Codes: A61P 3/06 Antihyperlipidemics
• CPC Class Codes: A61P 3/10 : for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 39/06 : Free radical scavengers or antioxidants
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: C12N 1/00 : Microorganisms, e.g. protozoa; Compositions thereof medicinal preparations containing material from protozoa, bacteria or viruses A61K35/66, from algae A61K36/02, from fungi A61K36/06; preparing medicinal bacterial antigen or antibody compositions, e.g. bacterial vaccines, A61K39/00; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
• CPC Class Codes: C12P 7/6427 : Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
• CPC Class Codes: C12R 2001/00 : Microorganisms ; Processes using microorganisms

Abstract

Disclosed are compositions and methods related to eukaryotic microorganisms that can produce unsaturated fatty acids which can be purified and use.

Claims

1. An isolated eukaryotic microorganism having an 18S sequence, wherein the 18S sequence has at least 97% identity to the sequence set forth in SEQ ID NO:

1.

2. The isolated microorganism of claim 1, wherein the microorganism is from the order Thraustochytriales.

3. The isolated microorganism of claim 1, wherein the microorganism is from the family Thraustochytriaceae.

4. The isolated microorganism of claim 1, wherein the microorganism is from the genus Thraustochytrium.

5. The isolated microorganism of claim 1, wherein the microorganism is Thraustochytrium sp.

6. The isolated microorganism of claim 1, wherein the microorganism is Thraustochytrium aureum.

7. The isolated microorganism of claim 1, wherein the microorganism is Thraustochytrium roseum.

8. The isolated microorganism of claim 1, wherein the microorganism is Thraustochytrium striatum.

9. The isolated microorganism of claim 1, wherein the microorganism is from the genus Schizochytrium.

10. The isolated microorganism of claim 1, wherein the microorganism is Schizochytrium sp.

11. The isolated microorganism of claim 1, wherein the microorganism comprises an omega 3 or omega 6 fatty acid.

12. The isolated microorganism of claim 1, wherein the microorganism comprises docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or docosapentaenoic acid (DPA).

13. The isolated microorganism of claim 1, wherein the microorganism produces a lipid or fatty acid fraction of at least about 30 wt. % to 80 wt. %.

14. The isolated microorganism of claim 13, wherein the lipid comprises DHA.

15. The isolated microorganism of claim 13, wherein the lipid composition comprises from about 15 wt. % fatty acid fraction to about 60 wt. % fatty acid fraction of n-3 DHA, from about 4 wt. % fatty acid fraction to about 12 wt. % fatty acid fraction of n-6 DPA, or from about 0 wt. % fatty acid fraction to about 3 wt. % fatty acid fraction of n-3 EPA.

16. The isolated microorganism of claim 13, wherein the lipid composition comprises from about 15 wt. % fatty acid fraction to about 60 wt. % fatty acid fraction of n-3 DHA.

17. The microorganism of claim 1, wherein the microorganism is from the family Thraustochytriaceae and has ATCC accession number PTA-6245.

18. A method of preparing a lipid composition, the method comprising:

culturing the eukaryotic microorganism of claim 1, in a heterotrophic medium, and isolating the lipid composition from the microorganism.

19. An isolated mutant of the eukaryotic microorganism of claim 1, wherein the isolated mutant has an 18S sequence, wherein the 18S sequence has at least 97% identity to the sequence set forth in SEQ ID NO:

1.

20. The isolated microorganism of claim 1, wherein the 18S sequence has at least 98% identity to the sequence set forth in SEQ ID NO:

1.

21. The isolated microorganism of claim 1, wherein the 18S sequence has at least 99% identity to the sequence set forth in SEQ ID NO:

1.

22. An composition comprising an isolated eukaryotic microorganism having an 18S sequence, wherein the 18S sequence has at least 97% identity to the sequence set forth in SEQ ID NO:

1.

23. The composition of claim 22, wherein the isolated eukaryotic microorganism is from the family Thraustochytriaceae and has ATCC accession number PTA-6245.

24. A nutritional supplement comprising the composition of claim 22.