Device and method for multiple analyte detection

• US 8,163,538 B2
• Filed: 01/09/2007
• Issued: 04/24/2012
• Est. Priority Date: 04/03/1996
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Woudenberg, Timothy, Albin, Michael, Kowallis, Reid B., Raysberg, Yefim, Ragusa, Robert P., Winn-Deen, Emily S.
• Primary Examiner(s): Yu, Melanie J
• Application Number:
  US11/651,321
• Publication Number:
  US 20070111300A1
• Time in Patent Office: 1,932 Days
• Field of Search: 436/809, 436/807, 436/808, 436/518, 435/7.1, 435/283.1, 435/287.2, 435/288.3, 435/288.4, 435/288.5, 435/288.7
• US Class Current: 435/288.5
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/08 using multifield media
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/08 Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/808 : Automated or kit
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

Claims

1. A method for analyzing a fluid sample, the method comprising:

filling with the fluid sample a substrate comprising a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel providing fluid connections between each of said two or more detection chambers and said inlet, wherein at least two of said two or more detection chambers each contains detection reagents comprising a different selected-sequence polynucleotide pre-loaded therein, and each selected-sequence polynucleotide is effective to selectively bind to a respective target polynucleotide, the filling comprising filling the fluid sample into the two or more detection chambers until all of the two or more detection chambers are filled with the fluid sample and the filling is stopped;

after all of the two or more detection chambers are filled with the fluid sample and the filling is stopped, then isolating the two or more detection chambers from one another such that the fluid sample is prevented from flowing from one of the detection chambers to another of the detection chambers;

reacting the fluid sample with the detection reagents in the two or more detection chambers;

producing detection signals in the two or more detection chambers; and

detecting the detection signals produced in the two or more detection chambers.

2. The method of claim 1, wherein the fluid connections comprise dead-end fluid connections.

3. The method of claim 1, wherein the filling with the fluid sample comprises filling a first detection chamber with the fluid sample and then flowing the fluid sample through the channel without disturbing the contents of the first detection chamber.

4. The method of claim 3, wherein the fluid connections comprise dead-end fluid connections and the producing detection signals comprises avoiding producing erroneous signals in the two or more detection chambers.

5. The method of claim 1, wherein the isolating comprises clearing the channel of the fluid sample after the filling with the fluid sample.

6. The method of claim 1, further comprising removing the fluid sample from the channel after the filling with the fluid sample.

7. The method of claim 6, wherein the substrate comprises a port in fluid communication with the channel and the removing comprises removing the fluid sample through the port.

8. The method of claim 1, wherein the producing detection signals comprises producing optical detection signals.

9. The method of claim 1, wherein the producing detection signals comprises producing fluorescent optical signals.

10. The method of claim 9, wherein the detection reagents comprise fluorescent detection reagents.

11. The method of claim 1, wherein the substrate comprises one or more layers comprising a heat-conducting metal material, and the reacting comprises heating the detection chambers.

12. The method of claim 11, wherein the one or more layers comprising a heat-conducting metal material comprises a heat-conducting metal coated with a plastic polymer.

13. The method of claim 1, wherein the substrate comprises at least one optically transparent window, the detecting the detection signals comprises detecting optical signals, and the detecting the optical signals comprises detecting through the at least one optically transparent window.

14. The method of claim 13, wherein the at least one optically transparent window comprises an optically transparent window for each detection chamber.

15. The method of claim 13, wherein the detecting comprises measuring wavelengths of interest and the at least one optically transparent window comprises an optically transparent material having low fluorescence emission at the wavelengths of interest and exhibiting minimal light absorption for the wavelengths of interest.

16. The method of claim 1, wherein the substrate comprises at least one optically transparent window, the detecting comprises detecting through the at least one optically transparent window, and the at least one optically transparent window comprises an optically transparent plastic layer.

17. The method of claim 1, wherein the detecting comprises detecting with a non-optical sensor.

18. The method of claim 1, wherein the reacting, the producing, and the detecting occur in the detection chambers after the isolating, and wherein after the isolating, fluid sample is prevented from flowing from one of the detection chambers to another of the detection chambers.

19. The method of claim 1, wherein the filling comprises dividing the fluid sample into sample portions each contained within respective sample chambers, and wherein the reacting, the producing, and the detecting occur in one of the sample chambers for a respective sample portion.