Photon reducing agents for fluorescence assays
First Claim
1. A method of reducing undesired light emission from a sample, comprising:
- contacting a sample in need of reducing undesired light with at least one photon reducing agent,wherein said at least one photon reducing agent is substantially membrane impermeant,wherein said sample comprises a membrane compartment,wherein said membrane compartment includes at least one photon producing agent and said at least one photon reducing agent is in an aqueous solution that contacts an outer surface of said membrane compartment,wherein said at least one photon reducing agent does not specifically bind to said membrane compartment, anddetecting an optical signal from said at least one photon producing agent.
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Abstract
The present invention provides a method for reducing undesirable light emission from a sample using at least one photon producing agent and at least one photon reducing agent (e.g. dye-based photon reducing agents). The present invention further provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one collisional quencher. The present invention also provides a method for reducing undesirable light emission from a sample (e.g., a biochemical or cellular sample) with at least one photon producing agent and at least one quencher, such as an electronic quencher. The present invention also provides a system and method of screening test chemicals in fluorescent assays using photon reducing agents. The present invention also provides compositions, pharmaceutical compositions, and kits for practicing these methods.
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Citations
19 Claims
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1. A method of reducing undesired light emission from a sample, comprising:
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contacting a sample in need of reducing undesired light with at least one photon reducing agent, wherein said at least one photon reducing agent is substantially membrane impermeant, wherein said sample comprises a membrane compartment, wherein said membrane compartment includes at least one photon producing agent and said at least one photon reducing agent is in an aqueous solution that contacts an outer surface of said membrane compartment, wherein said at least one photon reducing agent does not specifically bind to said membrane compartment, and detecting an optical signal from said at least one photon producing agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A composition of matter, comprising:
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a) a membrane compartment, wherein said membrane compartment comprises at least one photon producing agent, and b) an aqueous solution with at least one photon reducing agent, wherein said aqueous solution is in contact with an outer surface of said membrane compartment, wherein said at least one photon reducing agent is substantially membrane impermeant and does not specifically bind to said membrane compartment, wherein said at least one photon reducing agent has an absorption spectrum that overlaps with the absorption, emission or excitation spectrum of said at least one photon producing agent, and wherein said at least one photon reducing agent is present in said aqueous solution at a concentration sufficient to reduce light emitted from said aqueous solution by at least 10% compared to the light emitted of said aqueous solution in the absence of said at least one photon reducing agent. - View Dependent Claims (16, 17, 18, 19)
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Specification