Methods and products for analyzing polymers

• US 8,168,380 B2
• Filed: 05/10/2001
• Issued: 05/01/2012
• Est. Priority Date: 02/12/1997
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Chan, Eugene Y.
• Primary Examiner(s): MUMMERT, STEPHANIE KANE
• Application Number:
  US09/852,968
• Publication Number:
  US 20020119455A1
• Time in Patent Office: 4,009 Days
• Field of Search: None
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/6432 Quenching
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/6441 with two or more labels
• CPC Class Codes: G01N 2021/6482 Sample cells, cuvettes
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 33/48721 Investigating individual macromolecules, e.g. by translocation through nanopores Coulter counters in general G01N15/12; fabrication methods for nanoscale apertures B81B1/00; sequencing of nucleic acids C12Q1/68
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte

Abstract

Methods and products for analyzing polymers are provided. The methods include methods for determining various other structural properties of the polymers.

Claims

1. A method comprising:

providing a nucleic acid, labeled with a unit specific marker, and

detecting, while the nucleic acid is moved relative to electromagnetic radiation by a tethered polymerase, at least one FRET-independent fluorescent signal emitted from the unit specific marker.

3. The method of claim 1, wherein the electromagnetic radiation is transported through a waveguide.

5. The method of claim 1, further including storing signatures of signals.

6. The method of claim 5, wherein the electromagnetic radiation is transported through a waveguide.

2. A method for detecting a unit specific marker bound to a nucleic acid comprising

moving a nucleic acid relative to electromagnetic radiation using a tethered polymerase,

exposing a fluorescently labeled unit specific marker bound to the nucleic acid to electromagnetic radiation, and

detecting an electromagnetic radiation signal from the fluorescently labeled unit specific marker in the absence of fluorescence resonance energy transfer.

4. The method of claim 2, wherein the electromagnetic radiation is transported through a waveguide.