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Methods and compositions for tagging and identifying polynucleotides

  • US 8,168,385 B2
  • Filed: 08/16/2011
  • Issued: 05/01/2012
  • Est. Priority Date: 02/10/2005
  • Status: Active Grant
First Claim
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1. A method of sample analysis, comprising:

  • a) separately ligating an oligonucleotide tag to each of a plurality of samples that comprise fragmented genomic DNA to produce a plurality of adaptor-ligated samples, wherein;

    i. each of said samples is made from a different source;

    ii. each of said samples is ligated to a different oligonucleotide tag;

    iii. each oligonucleotide tag comprises at least one word comprising a unique nucleotide sequence that distinguishes said oligonucleotide tag from other oligonucleotide tags; and

    iv. each adaptor-ligated sample comprises adaptor-ligated genomic fragments that contain an adaptor and a genomic fragment;

    b) pooling said plurality of adaptor-ligated samples to make a pooled sample;

    c) isolating from said pooled sample adaptor-ligated genomic fragments that correspond to one or more genomic loci, thereby producing isolated fragments; and

    d) sequencing an isolated fragment of (c) to obtain;

    i. the unique nucleotide sequence of an oligonucleotide tag; and

    ii. the nucleotide sequence of at least part of a genomic fragment;

    wherein the unique nucleotide sequence of said isolated fragment identifies from which of said plurality of different polynucleotide samples said isolated fragment is derived.

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