Instrument for cassette for sample preparation

• US 8,168,443 B2
• Filed: 09/16/2011
• Issued: 05/01/2012
• Est. Priority Date: 12/27/2006
• Status: Active Grant

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Genturadx, Inc.
• Inventors: Yu, Steve Jia Chang, Ching, Jesus, Lee, Phillip You Fai, Hu, David Hsiang
• Primary Examiner(s): Bullock, In Suk
• Assistant Examiner(s): Wecker, Jennifer
• Application Number:
  US13/234,770
• Publication Number:
  US 20120003631A1
• Time in Patent Office: 228 Days
• Field of Search: None
• US Class Current: 436/174
• CPC Class Codes: B01F 35/7174 using pistons, plungers or syringes
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/023 Sending and receiving of information, e.g. using bluetooth
• CPC Class Codes: B01L 2300/0609 Holders integrated in container to position an object
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/18 Means for temperature control
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: B01L 3/527 for a plurality of reagents
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/00366 Several different temperatures used
• CPC Class Codes: G01N 2035/00544 using fluid flow
• CPC Class Codes: G01N 2035/00574 Means for distributing beads
• CPC Class Codes: G01N 2035/00801 : Holders for sample carriers, e.g. trays, caroussel, racks
• CPC Class Codes: G01N 2035/00811 : consumable or exchangeable components other than sample carriers, e.g. detectors, flow cells
• CPC Class Codes: G01N 2035/00881 : network configurations
• CPC Class Codes: G01N 2035/0091 : GUI [graphical user interfaces]
• CPC Class Codes: G01N 2035/0412 : Block or rack elements with a single row of samples
• CPC Class Codes: G01N 2035/0415 : moving in two dimensions in a horizontal plane
• CPC Class Codes: G01N 35/00871 : Communications between instruments or with remote terminals
• CPC Class Codes: G01N 35/0098 : involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/026 : having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: G01N 35/028 : having reaction cells in the form of microtitration plates
• CPC Class Codes: G01N 35/04 : Details of the conveyor system G01N35/021 - G01N35/028 take precedence
• CPC Class Codes: G01N 35/08 : using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10T 436/10 : Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
• CPC Class Codes: Y10T 436/11 : Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 : with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/115831 : Condition or time responsive
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.

Claims

1. A method, comprising:

disposing a plurality of cassettes into an instrument enclosure, each cassette from the plurality of cassettes including a valve member defining a cavity within which a particle can be disposed, the valve member configured to transfer the particle between a first chamber of the cassette and a second chamber of the cassette, each cassette from the plurality of cassettes defining a first reagent chamber and a second reagent chamber;

moving a plurality of valve shafts between a first position and a second position to apply a magnetic force produced by a magnetic portion of each valve shaft to attract the particle into the cavity of the valve member of the corresponding cassette from the plurality of cassettes;

rotating each valve shaft from the plurality of valve shafts when the plurality of valve shafts is in the second position to transfer the particle between the first chamber of the cassette and the second chamber of the cassette;

moving a plurality of protrusions between a first protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the first reagent chamber from the corresponding cassette and a second protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the second reagent chamber from the corresponding cassette; and

moving, when the plurality of protrusions is in the first protrusion position, a protrusion from the plurality of protrusions into contact with a plunger associated with the first reagent chamber of the corresponding cassette to transfer a first reagent from the first reagent chamber into the first chamber.

2. The method of claim 1, further comprising:

moving, when the plurality of protrusions is in the second protrusion position, the protrusion from the plurality of protrusions into contact with a plunger associated with the second reagent chamber of the corresponding cassette to transfer a second reagent from the second reagent chamber into the first chamber.

3. The method of claim 1, wherein:

the moving a plurality of protrusions between the first protrusion position and the second protrusion position includes moving the plurality of protrusions along a first axis; and

the moving the protrusion from the plurality of protrusions into contact with the plunger includes moving the protrusion along a second axis.

4. The method of claim 1, wherein:

the moving the protrusion from the plurality of protrusions into contact with the plunger includes moving at least a portion of the protrusion from the plurality of protrusions into the first reagent chamber to transfer the first reagent from the first reagent chamber into the first chamber.

5. The method of claim 1, wherein:

each cassette from the plurality of cassettes includes a puncturable member disposed between the first chamber and the first reagent chamber; and

the moving the protrusion from the plurality of protrusions into contact with the plunger includes puncturing a portion of the puncturable member.

6. The method of claim 1, wherein each cassette from the plurality of cassettes includes a pump configured to mix at least one of the first reagent or the second reagent with the particle within the first chamber, the method further comprising:

actuating each pump each pump from the plurality of cassettes with the plurality of protrusions.

7. The method of claim 1, wherein the moving a plurality of valve shafts between the first position and the second position includes disposing the magnetic portion of each valve shaft into an opening defined by the valve member of the corresponding cassette from the plurality of cassettes.

8. A method, comprising:

disposing a plurality of cassettes into an instrument enclosure, each cassette from the plurality of cassettes including a valve member defining a cavity within which a particle can be disposed, the valve member configured to transfer the particle between a first chamber of the cassette and a second chamber of the cassette, each cassette from the plurality of cassettes including a reagent module defining a first reagent chamber and a second reagent chamber;

moving a plurality of valve shafts between a first position and a second position to apply a magnetic force produced by a magnetic portion of each valve shaft to attract the particle into the cavity of the valve member of the corresponding cassette from the plurality of cassettes;

rotating each valve shaft from the plurality of valve shafts when the plurality of valve shafts is in the second position to transfer the particle between the first chamber of the cassette and the second chamber of the cassette;

moving a plurality of protrusions to a first protrusion position to transfer a first reagent from the first reagent chamber into the first chamber, each protrusion from the plurality of protrusions is at least partially within the first reagent chamber from the corresponding cassette when the plurality of protrusions is in the first protrusion position; and

moving the plurality of protrusions to a second protrusion position to transfer a second reagent from the second reagent chamber into the first chamber, each protrusion from the plurality of protrusions is at least partially within the second reagent chamber from the corresponding cassette when the plurality of protrusions is in the second protrusion position.

9. The method of claim 8, wherein:

the reagent module of each cassette from the plurality of cassettes includes a puncturable member disposed between the first chamber and each of the first reagent chamber and the second reagent chamber such that the first chamber is fluidically isolated from each of the first reagent chamber and the second reagent chamber;

the moving the plurality of protrusions to the first protrusion position includes puncturing a first portion of the puncturable member from a reagent module from a cassette of the plurality of cassettes; and

the moving the plurality of protrusions to the second protrusion position includes puncturing a second portion of the puncturable member from the reagent module from the cassette of the plurality of cassettes.

10. The method of claim 8, wherein:

the moving the plurality of protrusions to the first protrusion position includes moving a plunger within each first reagent chamber of the corresponding cassette to transfer the first reagent from the first reagent chamber into the first chamber; and

the moving the plurality of protrusions to the second protrusion position includes moving a plunger within each second reagent chamber of the corresponding cassette to transfer the second reagent from the second reagent chamber into the first chamber.

11. The method of claim 8, wherein each cassette from the plurality of cassettes includes a pump configured to mix at least one of the first reagent or the second reagent with the particle within the first chamber, the method further comprising:

moving the plurality of protrusions to a third protrusion position to actuate each pump from the plurality of pumps.

12. The method of claim 8, further comprising:

moving a plurality of heating members from a first heating member position, in which each heating member from the plurality of heating members is disposed apart from a corresponding cassette from the plurality of cassettes, to a second heating member position, in which each heating member from the plurality of heating members is engaged with the corresponding cassette from the plurality of cassettes.

13. A method, comprising:

disposing a plurality of cassettes into an instrument enclosure, each cassette from the plurality of cassettes including a valve configured to transfer a particle between a first chamber of the cassette and a second chamber of the cassette while maintaining fluid isolation between the first chamber and the second chamber, each cassette from the plurality of cassettes defining a first reagent chamber and a second reagent chamber;

moving a plurality of valve shafts between a first position and a second position, in the first position each valve shaft from the plurality of valve shafts is disposed apart from a corresponding cassette from the plurality of cassettes, in the second position each valve shaft from the plurality of valve shafts is engaged with the valve of the corresponding cassette from the plurality of cassettes;

moving a plurality of protrusions between a first protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the first reagent chamber from the corresponding cassette and a second protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the second reagent chamber from the corresponding cassette; and

moving, when the plurality of protrusions is in the first protrusion position, a protrusion from the plurality of protrusions into contact with a plunger associated with the first reagent chamber of the corresponding cassette to transfer a first reagent from the first reagent chamber into the first chamber.

14. The method of claim 13, further comprising:

moving, when the plurality of protrusions is in the second protrusion position, the protrusion from the plurality of protrusions into contact with a plunger associated with the second reagent chamber of the corresponding cassette to transfer a second reagent from the second reagent chamber into the first chamber.

15. The method of claim 13, wherein:

the moving a plurality of protrusions between the first protrusion position and the second protrusion position includes moving the plurality of protrusions along a first axis; and

the moving the protrusion from the plurality of protrusions into contact with the plunger includes moving the protrusion along a second axis.

16. The method of claim 13, wherein:

the moving the protrusion from the plurality of protrusions into contact with the plunger includes moving at least a portion of the protrusion from the plurality of protrusions into the first reagent chamber to transfer the first reagent from the first reagent chamber into the first chamber.

17. The method of claim 13, wherein:

each cassette from the plurality of cassettes includes a puncturable member disposed between the first chamber and the first reagent chamber; and

the moving the protrusion from the plurality of protrusions into contact with the plunger includes puncturing a portion of the puncturable member.

18. The method of claim 13, wherein each cassette from the plurality of cassettes includes a pump configured to mix at least one of the first reagent or the second reagent with the particle within the first chamber, the method further comprising:

actuating each pump each pump from the plurality of cassettes with the plurality of protrusions.

19. The method of claim 13, further comprising:

rotating each valve shaft from the plurality of valve shafts when the plurality of valve shafts is engaged with the valve of the corresponding cassette from the plurality of cassettes to transfer the particle from the first chamber of the cassette to the second chamber of the cassette.

20. The method of claim 13, wherein the valve of each cassette from the plurality of cassettes includes a valve member defining a cavity within which the particle is disposed when the particle is transferred between the first chamber of the cassette and the second chamber of the cassette, the method further comprising:

applying a magnetic force produced by a magnetic portion of each valve shaft to attract the particle into the cavity of the valve member of the corresponding cassette from the plurality of cassettes when the plurality of valve shafts is engaged with the valve of the corresponding cassette from the plurality of cassettes.