Methods for accumulating heterologous polypeptides in plant stover utilizing a vacuole sorting signal
First Claim
1. An isolated nucleic acid molecule comprising a protein storage vacuole targeting sequence operably linked to a nucleic acid encoding a cellobiohydrolase (CBH), which is operatively linked to a plant green tissue specific promoter, wherein the protein storage vacuole targeting sequence comprises the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO;
5.
1 Assignment
0 Petitions
Accused Products
Abstract
Compositions and methods for increasing the expression and/or accumulation of cellobiohydrolase enzyme in the vacuoles of plant cells are provided. The method involves targeting the enzyme to the vacuoles through the use of a barley polyamine oxidase (BPAO) vacuole sorting signal peptide. Plants transformed with an expression construct encoding the vacuole sorting signal peptide operably linked to the cellobiohydrolase enzyme direct expression of the polypeptide to the vacuoles of the plant cells. Transgenic plants, seeds, and plant tissues, and plant parts are provided. Downstream uses of transgenic plants or plant material expressing the constructs of the invention include agronomical and industrial uses, for example, human food, animal feed, biofuel, industrial alcohol, fermentation feedstocks, and the like.
-
Citations
26 Claims
-
1. An isolated nucleic acid molecule comprising a protein storage vacuole targeting sequence operably linked to a nucleic acid encoding a cellobiohydrolase (CBH), which is operatively linked to a plant green tissue specific promoter, wherein the protein storage vacuole targeting sequence comprises the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO;
5. - View Dependent Claims (2, 3, 4, 5)
- 2 or SEQ ID NO;
-
6. A method for accumulating a cellobiohydrolase (CBH) in a transgenic plant comprising the steps of:
-
a) operatively linking a plant green tissue specific promoter, a protein storage vacuole targeting sequence and a nucleic acid encoding the cellobiohydrolase, wherein the protein storage vacuole targeting sequence comprises the amino acid sequence of SEQ ID NO;
2 or SEQ ID NO;
5; andb) generating a transgenic plant wherein the cellobiohydrolase is targeted to the vacuole. - View Dependent Claims (7, 8, 9, 10)
-
-
11. A transgenic plant cell comprising a nucleic acid molecule comprising a polynucleotide encoding a protein storage vacuole targeting sequence operably linked to a nucleic acid encoding a cellobiohydrolase which is operatively linked to a plant green tissue specific promoter, wherein the protein storage vacuole targeting sequence comprises the amino acid sequence of SEQ ID NO:
- 2 or SEQ ID NO;
5. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18)
- 2 or SEQ ID NO;
-
19. A method for accumulating a cellobiohydrolase (CBH) in plant stover comprising the steps of;
-
a) introducing into a plant cell a nucleic acid construct comprising a polynucleotide encoding a protein storage vacuole sorting signal sequence and a plant green tissue specific promoter, each of which is operably linked to a nucleotide sequence encoding a cellobiohydrolase, wherein the protein storage vacuole sorting signal sequence comprises the amino acid sequence of SEQ ID NO;
2 or SEQ ID NO;
5;b) regenerating a transformed plant from said plant cell; and c) allowing the transformed plant to senesce to form stover, thereby accumulating the cellobiohydrolase in said plant stover. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26)
-
Specification